高效液相色谱法测定尿液中雌三醇和肌酐的含量。

Determination of estriol and creatinine in urine by high performance liquid chromatography.

作者信息

Ke L, Yuan Y S

机构信息

Department of Instrumental Analysis, Jinling Hospital, Nanjing, The People's Republic of China.

出版信息

Biomed Chromatogr. 1989 Sep;3(5):196-8. doi: 10.1002/bmc.1130030504.

DOI:10.1002/bmc.1130030504

PMID:2804424

Abstract

A quantitative method for the determination of estriol (E3) and creatinine (C) in random urine by high performance liquid chromatography is described. The mobile phase was a mixed solution of methanol and phosphate buffer (0.025 M, pH 6.5) and the detection wavelength was at 205 nm. The method was simple, rapid and accurate. The OCV for E3 and C using this method were 1.7-3.4% and 2.2-2.5%, respectively. The RCV for E3 and C were 6.2-7.0% and 4.5-6.9%, respectively. The recoveries were 87-104% for E3 and 98-103% for C, respectively. The method has been used for clinical determinations.

摘要

描述了一种用高效液相色谱法测定随机尿中雌三醇（E3）和肌酐（C）的定量方法。流动相为甲醇和磷酸盐缓冲液（0.025M，pH 6.5）的混合溶液，检测波长为205nm。该方法简单、快速且准确。用此方法测定E3和C的OCV分别为1.7 - 3.4%和2.2 - 2.5%。E3和C的RCV分别为6.2 - 7.0%和4.5 - 6.9%。E3的回收率为87 - 104%，C的回收率为98 - 103%。该方法已用于临床检测。