Sano A, Takezawa M, Takitani S
Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
Biomed Chromatogr. 1989 Sep;3(5):209-12. doi: 10.1002/bmc.1130030507.
A method for the determination of cyanide in human urine has been developed. The method is based on the reaction of cyanide with 2,3-naphthalenedialdehyde and taurine to give a fluorescent product for reversed-phase HPLC separation and fluorometric detection. After centrifugation followed by dilution of urine samples, the specimens could be analysed directly by this method. The recovery of cyanide added to urine at concentration levels of 50-1000 pmol/mL was 85-96%. The detection limit of cyanide was 30 pmol/mL in urine. The method was successfully applied to the analysis of urine from smokers and nonsmokers. The mean concentrations of cyanide were found to be 215 pmol/mL for the former and 84 pmol/mL for the latter.
已开发出一种测定人尿中氰化物的方法。该方法基于氰化物与2,3 -萘二醛和牛磺酸反应生成荧光产物，用于反相高效液相色谱分离和荧光检测。尿样经离心后稀释，即可直接用该方法进行分析。向尿样中添加浓度为50 - 1000 pmol/mL的氰化物，回收率为85 - 96%。尿中氰化物的检测限为30 pmol/mL。该方法成功应用于吸烟者和非吸烟者尿液的分析。结果发现，前者氰化物的平均浓度为215 pmol/mL，后者为84 pmol/mL。
