Sano A, Takimoto N, Takitani S
Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
J Chromatogr. 1992 Nov 6;582(1-2):131-5. doi: 10.1016/0378-4347(92)80311-d.
A method for the determination of cyanide in human red cells has been developed. Cyanide was extracted from red cells by adding water and methanol, and then derivatized with 2,3-naphthalene-dialdehyde and taurine to give a fluorescent product, which was determined by reversed-phase high-performance liquid chromatography with fluorescence detection. The recovery of cyanide from red cells was ca. 83%, and the limit of detection was 100 pmol/ml. The mean concentrations of red cell cyanide from ten smokers and from ten non-smokers were 705 and 466 pmol/ml, respectively. The method was also applicable to whole blood.
