Giachetti C, Zanolo G, Assandri A, Poletti P
Biomedical Research Institute, Ivrea, Italy.
Biomed Environ Mass Spectrom. 1989 Aug;18(8):592-7. doi: 10.1002/bms.1200180814.
A gas chromatographic/mass spectrometric analytical procedure is described to determine glycols in plasma as cyclic butyl boronate esters. The method, involving a pre-deproteinization step, required only 0.25 ml of plasma and a short time (20 min) to react with the derivatizing agent (butyl boronic acid). The gas chromatographic separation on a CP Sil 8 CB silica capillary column coupled to a mass detector assured a complete identification of the compounds. The analytical recoveries (greater than 95%) with low coefficient of variation (4-11%) assured the feasibility of the method over a concentration range from 5 to 1000 micrograms ml-1 for each glycol. The lower detection limits, namely 1-5 micrograms ml-1, confirmed the sensitivity of the method.
描述了一种气相色谱/质谱分析方法，用于将血浆中的二醇测定为环丁基硼酸酯。该方法包括预脱蛋白步骤，仅需0.25 ml血浆，且与衍生化试剂（丁基硼酸）反应时间短（20分钟）。在与质量检测器相连的CP Sil 8 CB硅胶毛细管柱上进行气相色谱分离，确保了化合物的完全鉴定。分析回收率（大于95%）和低变异系数（4 - 11%）确保了该方法在每种二醇5至1000微克/毫升的浓度范围内的可行性。较低的检测限，即1 - 5微克/毫升，证实了该方法的灵敏度。
