Cui Pengfei, Guo Wenbin, Chen Xinhua
a Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography , State Oceanic Administration , Xiamen , P.R. China.
b Key Laboratory of Marine Genetic Resources of Fujian Province , Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources , Xiamen , P.R. China.
Nat Prod Res. 2017 Sep;31(18):2153-2157. doi: 10.1080/14786419.2016.1274896. Epub 2017 Jan 3.
Strain Pc3 was isolated from Antarctic seawater and identified as Bacillus amyloliquefaciens. A compound with antifungal activity was purified from the fermentation supernatant of B. amyloliquefaciens Pc3. Its structure was determined to be (S)-2-amino-3-(1H-indol-2-yl) propanoic acid, named as isotryptophan, based on detailed analysis of its nuclear magnetic resonance (NMR) and high-resolution electrospray ionisation mass spectrometry (HR-ESI-MS). Isotryptophan exhibited good thermal stability and antifungal activity against several plant-pathogenic fungi with low minimum inhibitory concentrations. Therefore, the characterisation of isotryptophan from the Antarctic B. amyloliquefaciens Pc3 will facilitate its potential application in the control of plant-pathogenic fungal infection.
菌株Pc3从南极海水中分离出来，被鉴定为解淀粉芽孢杆菌。从解淀粉芽孢杆菌Pc3的发酵上清液中纯化出一种具有抗真菌活性的化合物。基于对其核磁共振（NMR）和高分辨率电喷雾电离质谱（HR-ESI-MS）的详细分析，确定其结构为（S）-2-氨基-3-（1H-吲哚-2-基）丙酸，命名为异色氨酸。异色氨酸表现出良好的热稳定性，对几种植物病原真菌具有抗真菌活性，最低抑菌浓度较低。因此，对南极解淀粉芽孢杆菌Pc3中的异色氨酸进行表征将有助于其在控制植物病原真菌感染方面的潜在应用。
