Hugon J S, Ibarra C, Valenti G, Bourguet J
Centre de Biologie Cellulaire, CNRS, Ivry-sur-Seine, France.
Biol Cell. 1989;66(1-2):77-84.
Microtubules and microfilaments were localized by an immunocytochemical method in the granular cells of the frog bladder after fixation and isolation. An extensive array of microtubules was observed in the granular cells with an orientation towards the luminal plasma membrane in the supranuclear zone. Actin filaments formed a continuous bundle that underlined the cellular membrane. After incubation in the presence of colchicine, nocodazole, or tubulozole, the microtubular network appeared fragmented but did not disappear completely. These observations are related to the role of the cytoskeleton in the permeability response of the frog bladder epithelium to vasopressin.
采用免疫细胞化学方法，在固定和分离后的蛙膀胱颗粒细胞中定位微管和微丝。在颗粒细胞中观察到大量微管，其在核上区朝向管腔质膜定向排列。肌动蛋白丝形成连续的束，位于细胞膜下方。在秋水仙碱、诺考达唑或微管唑存在下孵育后，微管网络出现断裂但并未完全消失。这些观察结果与细胞骨架在蛙膀胱上皮对血管加压素的通透性反应中的作用有关。
