Center for Proteomics, State Key Laboratory of Bio-Control, MOE Key Lab Aquat Food Safety, School of Life Sciences, Guangdong Province Key Laboratory for Pharmaceutical Functional Genes, Sun Yat-sen University, University City, Guangzhou 510006, People's Republic of China.
Sci Rep. 2017 Jan 3;7:39824. doi: 10.1038/srep39824.
The occurrence of infectious diseases is related to heterogeneous protein interactions between a host and a microbe. Therefore, elucidating the host-pathogen interplay is essential. We previously revealed the protein interactome between Edwardsiella piscicida and fish gill cells, and the present study identified the protein interactome between E. piscicida and E. drummondhayi liver cells. E. drummondhayi liver cells and bacterial pull-down approaches were used to identify E. piscicida outer membrane proteins that bind to liver cells and fish liver cell proteins that interact with bacterial cells, respectively. Eight bacterial proteins and 11 fish proteins were characterized. Heterogeneous protein-protein interactions between these bacterial cells and fish liver cells were investigated through far-Western blotting and co-immunoprecipitation. A network was constructed based on 42 heterogeneous protein-protein interactions between seven bacterial proteins and 10 fish proteins. A comparison of the new interactome with the previously reported interactome showed that four bacterial proteins overlapped, whereas all of the identified fish proteins were new, suggesting a difference between bacterial tricks for evading host immunity and the host strategy for combating bacterial infection. Furthermore, these bacterial proteins were found to regulate the expression of host innate immune-related proteins. These findings indicate that the interactome contributes to bacterial infection and host immunity.
传染病的发生与宿主和微生物之间的异质蛋白相互作用有关。因此，阐明宿主-病原体相互作用至关重要。我们之前揭示了爱德华氏菌和鱼类鳃细胞之间的蛋白质互作组，本研究鉴定了爱德华氏菌和鱼类肝脏细胞之间的蛋白质互作组。使用鱼类肝脏细胞和细菌下拉方法，分别鉴定与肝脏细胞结合的爱德华氏菌外膜蛋白和与细菌细胞相互作用的鱼类肝脏细胞蛋白。鉴定了 8 种细菌蛋白和 11 种鱼类蛋白。通过远 Western 印迹和共免疫沉淀研究了这些细菌细胞和鱼类肝脏细胞之间的异质蛋白-蛋白相互作用。基于 7 种细菌蛋白和 10 种鱼类蛋白之间的 42 种异质蛋白-蛋白相互作用构建了一个网络。将新的互作组与之前报道的互作组进行比较，发现有 4 种细菌蛋白重叠，而所有鉴定出的鱼类蛋白都是新的，这表明细菌逃避宿主免疫的策略和宿主对抗细菌感染的策略存在差异。此外，这些细菌蛋白被发现调节宿主固有免疫相关蛋白的表达。这些发现表明互作组有助于细菌感染和宿主免疫。
