School of Physical Science and Technology, Xinjiang University, Urumqi 830046, China.
College of Information Science and Engineering, Xinjiang University, Urumqi 830046, China.
Sensors (Basel). 2017 Jan 1;17(1):80. doi: 10.3390/s17010080.
N-type macroporous silicon microcavity structures were prepared using electrochemical etching in an HF solution in the absence of light and oxidants. The CdSe/ZnS water-soluble quantum dot-labeled DNA target molecules were detected by monitoring the microcavity reflectance spectrum, which was characterized by the reflectance spectrum defect state position shift resulting from changes to the structures' refractive index. Quantum dots with a high refractive index and DNA coupling can improve the detection sensitivity by amplifying the optical response signals of the target DNA. The experimental results show that DNA combined with a quantum dot can improve the sensitivity of DNA detection by more than five times.
采用无光照和氧化剂的 HF 溶液电化学腐蚀法制备了 N 型大孔硅微腔结构。通过监测微腔反射谱来检测 CdSe/ZnS 水溶性量子点标记的 DNA 靶分子，其特征是由于结构折射率的变化导致反射谱缺陷态位置发生位移。高折射率的量子点和 DNA 偶联可以通过放大目标 DNA 的光学响应信号来提高检测灵敏度。实验结果表明，与量子点结合的 DNA 可以将 DNA 检测的灵敏度提高 5 倍以上。
