Lin V S, Motesharei K, Dancil K P, Sailor M J, Ghadiri M R
Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.
Science. 1997 Oct 31;278(5339):840-3. doi: 10.1126/science.278.5339.840.
A biosensor has been developed based on induced wavelength shifts in the Fabry-Perot fringes in the visible-light reflection spectrum of appropriately derivatized thin films of porous silicon semiconductors. Binding of molecules induced changes in the refractive index of the porous silicon. The validity and sensitivity of the system are demonstrated for small organic molecules (biotin and digoxigenin), 16-nucleotide DNA oligomers, and proteins (streptavidin and antibodies) at pico- and femtomolar analyte concentrations. The sensor is also highly effective for detecting single and multilayered molecular assemblies.
基于适当衍生化的多孔硅半导体薄膜可见光反射光谱中法布里-珀罗条纹的诱导波长偏移，开发了一种生物传感器。分子的结合引起了多孔硅折射率的变化。该系统对皮摩尔和飞摩尔分析物浓度下的小分子（生物素和地高辛）、16核苷酸DNA寡聚物和蛋白质（链霉亲和素和抗体）的有效性和灵敏度得到了证明。该传感器对于检测单层和多层分子组装体也非常有效。
