Che Yanli, Gui Shunhua, Lo Nathan, Ritchie Andrew, Wang Zongqing
College of Plant Protection, Southwest University, Beibei, Chongqing, P. R. China.
School of Life and Environmental Sciences, University of Sydney, Sydney, NSW, Australia.
PLoS One. 2017 Jan 3;12(1):e0169006. doi: 10.1371/journal.pone.0169006. eCollection 2017.
We collected Ectobiidae cockroach specimens from 44 locations in the south of the Yangtze valley. We obtained 297 COI sequences specimens and carried out phylogenetic and divergence dating analyses, as well as species delimitation analysis using a General Mixed Yule Coalescent (GMYC) framework. The intraspecific and interspecific sequence divergence in Ectobiidae cockroaches ranged from 0.0 to 7.0% and 4.6 to 30.8%, respectively. GMYC analysis resulted in 53 (confidence interval: 37-65) entities (likelihood ratio = 103.63) including 14 downloaded species. The COI GMYC groups partly corresponded to the ectobiid species and 52 ectobiid species were delimited successfully based on the combination of GMYC result with morphological information. We used the molecular data and 6 cockroach fossil calibrations to obtain a preliminary estimate of the timescale of ectobiid evolution. The major subfamilies in the group were found to have diverged between ~125-110 Ma, and morphospecies pairs were found to have diverged ~10 or more Ma.
我们从长江流域以南的44个地点采集了球蠊科蟑螂标本。我们获得了297个细胞色素氧化酶亚基I（COI）序列标本，并进行了系统发育和分歧时间分析，以及使用广义混合尤尔合并（GMYC）框架的物种界定分析。球蠊科蟑螂的种内和种间序列分歧分别为0.0%至7.0%和4.6%至30.8%。GMYC分析产生了53个（置信区间：37 - 65）实体（似然比 = 103.63），包括14个已下载的物种。COI的GMYC组部分对应于球蠊科物种，并且基于GMYC结果与形态学信息的结合成功界定了52个球蠊科物种。我们使用分子数据和6个蟑螂化石校准来初步估计球蠊科进化的时间尺度。发现该类群中的主要亚科在约1.25 - 1.10亿年前分化，形态物种对在约1000万年前或更早分化。
