Zhang J, Chen J H, Liu X D, Wang H Y, Liu X L, Li X Y, Wu Z F, Zhu M J, Zhao S H
J Anim Sci. 2016 Dec;94(12):5028-5041. doi: 10.2527/jas.2016-0924.
It has been shown that hematological traits can act as important indicators of immune function in both humans and livestock. T lymphocytes are key components of the adaptive immune system, playing a critical role in immune response. To identify genomic regions affecting hematological traits and T lymphocyte subpopulations, we performed both a SNP-based genomewide association study (GWAS) and a haplotype analysis for 20 hematological traits and 8 T cell subpopulations at 3 different time points (d 20, 33, and 35) in a Duroc × Erhualian F intercross population. Bonferroni correction was used to calculate the threshold -values for suggestive and 5% genomewide significance levels. In total, for SNP-based GWAS, we detected 96 significant SNP, including 15 genomewide-significant SNP, associated with 23 hematological traits and 234 significant SNP, including 27 genomewide-significant SNP, associated with 8 T cell subpopulations. Meanwhile, we identified 563 significant SNP, including 7 genomewide-significant SNP, associated with 5 hematological traits and 2,407 significant SNP, including 1,261 genomewide-significant SNP, associated with 8 T cell subpopulations by haplotype analysis. Among the significant regions detected, we propose both the () gene and the () gene on SSC3 as plausible candidate genes associated with CD/CD T lymphocytes at d 20. The findings provide insights into the basis of molecular mechanisms that are involved with immune response in the domestic pig and would aid further identification of causative mutations.
研究表明，血液学特征可作为人类和家畜免疫功能的重要指标。T淋巴细胞是适应性免疫系统的关键组成部分，在免疫反应中起关键作用。为了鉴定影响血液学特征和T淋巴细胞亚群的基因组区域，我们在杜洛克×二花脸F2代杂交群体中，针对20个血液学特征和8个T细胞亚群在3个不同时间点（第20、33和35天）进行了基于单核苷酸多态性（SNP）的全基因组关联研究（GWAS）和单倍型分析。采用Bonferroni校正来计算提示性和全基因组5%显著性水平的阈值。总体而言，对于基于SNP的GWAS，我们检测到96个显著SNP，包括15个全基因组显著SNP，与23个血液学特征相关；以及234个显著SNP，包括27个全基因组显著SNP，与8个T细胞亚群相关。同时，通过单倍型分析，我们鉴定出563个显著SNP，包括7个全基因组显著SNP，与5个血液学特征相关；以及2407个显著SNP，包括1261个全基因组显著SNP，与8个T细胞亚群相关。在检测到的显著区域中，我们提出位于猪3号染色体（SSC3）上的（）基因和（）基因是与第20天的CD4+/CD8+ T淋巴细胞相关的可能候选基因。这些发现为家猪免疫反应所涉及的分子机制基础提供了见解，并将有助于进一步鉴定致病突变。
