Hamed Arafa I, Ben Said Ridha, Al-Ayed Abdullah S, Moldoch Jaroslaw, Mahalel Usama A, Mahmoud Ayman M, Elgebaly Hassan A, Perez Andy J, Stochmal Anna
a Phytochemistry Laboratory, Department of Botany , Faculty of Science , Aswan University , Aswan , Egypt.
b Department of Chemistry , College of Science & Arts at Al-Rass, Qassim University , Kingdom of Saudi Arabia.
Nat Prod Res. 2017 Sep;31(17):2024-2031. doi: 10.1080/14786419.2016.1274887. Epub 2017 Jan 4.
A fingerprint of steroid saponins, the major constituent in 80% methanolic fraction from the male flowers of Phoenix dactylifera has been established. Under ESI-MS/MS conditions, the fragmentation patterns of [M - H] ions exclusively displayed signals corresponding to the cleavage of the glycosidic bonds, thus allowing a rapid identification of 21 steroidal saponins. Moreover, two unique among them conjugated with histidine were detected by LC-ESI (-)-MS and DFT and were given tentative names of 3-o-histidine-26-o-hexosyl-dioscin and 3-o-histidine-26-o-dihexosyl-hydroxydioscin. Their steroidal saponins exhibited a significant improvement of the sperm cells count, motility and viability in male rats. These effects could be attributed to enhancing the levels of sex hormones.
已确定海枣雄花80%甲醇提取物中的主要成分甾体皂苷的指纹图谱。在电喷雾串联质谱（ESI-MS/MS）条件下，[M - H]离子的碎裂模式仅显示对应糖苷键断裂的信号，从而能够快速鉴定出21种甾体皂苷。此外，通过液相色谱 - 电喷雾负离子质谱（LC-ESI (-)-MS）和密度泛函理论（DFT）检测到其中两种与组氨酸共轭的独特化合物，并暂定名为3-o-组氨酸-26-o-己糖基薯蓣皂苷和3-o-组氨酸-26-o-二己糖基羟基薯蓣皂苷。它们的甾体皂苷对雄性大鼠的精子细胞数量、活力和生存能力有显著改善。这些作用可能归因于性激素水平的提高。
