Madole Mahesh Basavaraj, Iyer Chandrashekhar M, Madivalar Mamatha Thimmanna, Wadde Satish Kishanrao, Howale Deepak Sadashiv
Associate Professor, Department of Biochemistry, GMERS , Medical College, Valsad, Gujarat, India .
Professor, Department of Biochemistry, Indira Gandhi Medical College , Nagpur, Maharashtra, India .
J Clin Diagn Res. 2016 Nov;10(11):BC01-BC04. doi: 10.7860/JCDR/2016/23787.8900. Epub 2016 Nov 1.
Diabetes mellitus (DM), a metabolic disorder characterized by hyperglycaemia, associated with deficiency or resistance to insulin indicates endocrinal abnormality of the pancreas. Amylase and lipase are enzymes secreted by the exocrine portion of the pancreas. Endocrinal derangement observed in diabetes may interfere with the exocrine function of the pancreas.
To estimate the levels of fasting blood sugar, serum lipase, serum amylase in patients of type 1 and type 2 DM. Than comparing them with healthy controls and to study the effect of type 1 and type 2 DM on pancreatic exocrine function using serum levels of amylase and lipase as biochemical marker.
This study was conducted at GMERS Medical College and Hospital from Dec 2015 to July 2016. Thirty patients of type 1 DM and 30 patients of type 2 DM, who were already diagnosed and taking treatment, were included in this study. A total number of 30 apparently healthy individuals were recruited as the control group in our study. Fasting venous blood samples were collected from the cases as well as the controls and they were analysed by using semi auto analyser for blood glucose, serum amylase and serum lipase. The results were analysed statistically by using SPSS software. Values were expressed as means ± SD.
We found statistically significant (p<0.01) low values for serum amylase and serum lipase in patients with type 1 and type 2 DM as compared to healthy controls. Fasting blood sugar was significantly higher in cases as compared to controls. We found negative correlation of fasting blood sugar level with serum amylase and serum lipase and positive correlation of serum amylase with serum lipase in both type 1 and type 2 DM.
Our study clearly demonstrated that in type 1 and type 2 DM, there was increase in fasting blood sugar with decrease in serum amylase and serum lipase which signifies the derangement of endocrine-exocrine axis of the pancreas. Serum amylase and serum lipase can be used as biochemical markers for assessment of pancreatic exocrine function.
糖尿病（DM）是一种以高血糖为特征的代谢紊乱疾病，与胰岛素缺乏或抵抗相关，提示胰腺内分泌异常。淀粉酶和脂肪酶是胰腺外分泌部分分泌的酶。糖尿病中观察到的内分泌紊乱可能会干扰胰腺的外分泌功能。
评估1型和2型糖尿病患者的空腹血糖、血清脂肪酶、血清淀粉酶水平。然后将它们与健康对照进行比较，并以血清淀粉酶和脂肪酶水平作为生化指标，研究1型和2型糖尿病对胰腺外分泌功能的影响。
本研究于2015年12月至2016年7月在GMERS医学院及医院进行。本研究纳入了30例已确诊并正在接受治疗的1型糖尿病患者和30例2型糖尿病患者。总共招募了30名明显健康的个体作为我们研究的对照组。从病例组和对照组采集空腹静脉血样本，使用半自动分析仪对血糖、血清淀粉酶和血清脂肪酶进行分析。结果使用SPSS软件进行统计学分析。数值以均值±标准差表示。
我们发现，与健康对照组相比，1型和2型糖尿病患者的血清淀粉酶和血清脂肪酶值在统计学上显著降低（p<0.01）。与对照组相比，病例组的空腹血糖显著更高。我们发现，在1型和2型糖尿病中，空腹血糖水平与血清淀粉酶和血清脂肪酶呈负相关，血清淀粉酶与血清脂肪酶呈正相关。
我们的研究清楚地表明，在1型和2型糖尿病中，空腹血糖升高，血清淀粉酶和血清脂肪酶降低，这表明胰腺内分泌-外分泌轴紊乱。血清淀粉酶和血清脂肪酶可作为评估胰腺外分泌功能的生化指标。
