单核细胞疗法对兔椎间盘退变的影响。

Influence of mononuclear cell therapy on disc degeneration in rabbits.

作者信息

Fávaro Rodrigo Caldonazzo, Arruda André de Oliveira, Vialle Luiz Roberto Gomes, Vialle Emiliano Neves

机构信息

Pontifícia Universidade Católica do Paraná, Hospital Universitário Cajuru, Serviço de Ortopedia e Traumatologia, Curitiba, PR, Brazil.

出版信息

Rev Bras Ortop. 2016 Oct 15;51(6):707-715. doi: 10.1016/j.rboe.2016.10.003. eCollection 2016 Nov-Dec.

DOI:10.1016/j.rboe.2016.10.003

PMID:28050544

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5198066/

Abstract

OBJECTIVE

The objective of this research was to evaluate the influence of autologous mononuclear stem cells injections on histological changes of collagen in the fibrous annulus of the intervertebral disk after experimental injury.

METHODS

32 New Zealand rabbits were submitted to intervertebral disk puncture, followed by intradiscal injection of mononuclear cells from the iliac crest saline injection in the following time periods: two months after the injury (SC2M and SS2M), two weeks (SC2W and SS2W) immediately after injury (SCCP and SSCP), and without inducing degeneration (SCSP and SSSP). Two months after cell therapy, the animals were euthanized and collagen changes in the intervertebral discs were histologically evaluated.

RESULTS

There were significant differences in ELAF between SS2W and SS2S groups ( = 0.018). This difference was due to an increase in type I collagen in SS2W group (56.7%) compared to SC2S (13.28%).

CONCLUSION

Treatment with mononuclear mesenchymal stem cells reduced changes in the type I and III collagen distribution in rabbits AF degenerated discs up to two weeks after the induction of degeneration.

摘要

目的

本研究的目的是评估自体单核干细胞注射对实验性损伤后椎间盘纤维环中胶原蛋白组织学变化的影响。

方法

32只新西兰兔接受椎间盘穿刺，随后在以下时间段进行椎间盘内注射来自髂嵴的单核细胞生理盐水注射：损伤后两个月（SC2M和SS2M）、损伤后两周（SC2W和SS2W）、损伤后立即（SCCP和SSCP），以及不诱导退变（SCSP和SSSP）。细胞治疗两个月后，对动物实施安乐死，并对椎间盘内的胶原蛋白变化进行组织学评估。