Zhang Baocai, Zhou Yihua
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Methods Mol Biol. 2017;1544:213-222. doi: 10.1007/978-1-4939-6722-3_15.
Gas chromatography-mass spectrometry (GC-MS) is a widely applied technique in carbohydrate composition analysis due to high accuracy and repeatability. As cell walls are the major carbohydrate pool in xylem tissues, the measurement of carbohydrate composition essentially constitutes an analysis of wall residues. Here, we describe the method for quantification of the contents of several common neutral monosaccharides and cellulose, the major components of cell walls in poplar xylem tissues, by GC-MS. The procedures for extraction of cell wall residues from xylem, generation of alditol derivatives, subsequent further separation on gas chromatograph, and detection by mass spectrometer are included.
气相色谱-质谱联用（GC-MS）由于具有高准确性和可重复性，是碳水化合物成分分析中广泛应用的技术。由于细胞壁是木质部组织中主要的碳水化合物库，碳水化合物成分的测量本质上构成了对细胞壁残余物的分析。在此，我们描述了通过GC-MS定量杨树木质部组织细胞壁中几种常见中性单糖和纤维素（细胞壁的主要成分）含量的方法。该方法包括从木质部中提取细胞壁残余物、生成糖醇衍生物、随后在气相色谱仪上进一步分离以及用质谱仪进行检测的步骤。
