Gilbart J, Fox A, Morgan S L
Department of Microbiology and Immunology, School of Medicine, University of South Carolina, Columbia 29208.
Eur J Clin Microbiol. 1987 Dec;6(6):715-23. doi: 10.1007/BF02013084.
Carbohydrate profiling by gas chromatography-mass spectrometry is a powerful tool for the identification and detection of bacteria. Its increasing applicability in the microbiology laboratory is illustrated by three examples. In the first, differentiation of legionellae by their sugar composition was determined with alditol acetate derivatization followed by selected- ion monitoring. In the second example, a carbohydrate pyrolysis product from Streptococcus agalactiae was used to differentiate group B streptococci from other Lancefield groups after direct sampling from culture plates. The third example employed the carbohydrates rhamnose and muramic acid as chemical markers for the direct detection of bacterial cell wall degradation products in mammalian tissues. The analysis of carbohydrate markers for bacteria by gas chromatography-mass spectrometry has great potential for use in clinical identification of isolated bacteria as well as in the rapid diagnosis of bacterial infections without prior culture.
气相色谱-质谱联用的碳水化合物谱分析是鉴定和检测细菌的有力工具。通过三个例子说明了其在微生物实验室中日益广泛的应用。第一个例子是，通过乙酰糖醇衍生化后进行选择离子监测，根据军团菌的糖组成来区分不同的军团菌。第二个例子是，从培养平板直接取样后，利用无乳链球菌的碳水化合物热解产物将B群链球菌与其他兰斯菲尔德菌群区分开来。第三个例子是，将鼠李糖和胞壁酸这两种碳水化合物用作化学标志物，直接检测哺乳动物组织中细菌细胞壁的降解产物。通过气相色谱-质谱联用分析细菌的碳水化合物标志物，在临床鉴定分离出的细菌以及在无需预先培养的情况下快速诊断细菌感染方面具有巨大的应用潜力。
