Madani Tariq A, Abuelzein El-Tayb M E, Jalalah Sawsan M, Abu-Araki Huda, Azhar Esam I, Hassan Ahmed M, Al-Bar Hussein M S
1 Department of Medicine, Faculty of Medicine, King Abdulaziz University , Jeddah, Saudi Arabia .
2 The Scientific Chair of Sheikh Mohammad Hussein Al-Amoudi for Viral Hemorrhagic Fever, King Abdulaziz University , Jeddah, Saudi Arabia .
Vector Borne Zoonotic Dis. 2017 Mar;17(3):195-199. doi: 10.1089/vbz.2016.2064. Epub 2017 Jan 4.
Alkhumra hemorrhagic fever virus (AHFV) is a newly described zoonotic flavivirus that was first isolated during 1994-1995 from the Alkhumra district south of Jeddah, Saudi Arabia. Subsequently, the virus was also isolated from Makkah city (2001-2003) and Najran (2008-2009), Saudi Arabia. The virus causes acute febrile illness with hepatitis, hemorrhagic manifestations, and encephalitis. A case fatality rate of 25% was reported among hospitalized patients. Although several biological and molecular characteristics of the virus have been published, no data are available on electron microscopic features of the virus. In this article, we describe the morphological features and metrics of the AHFV particles under electron microscopy, and localization of the virus particles in brain cells of newborn Wistar rats and in Rhesus monkey (Macaca mulatta) kidney epithelial cells (LLC-MK2). Virus particles in both the LLC-MK2 cells and the rat brain cells showed dark hexagonal core (capsid) and a translucent envelope. The mean diameter of the enveloped virus particle was 40.59 ± 1.29 nm in the rat brain cells (n = 154) and 40.97 ± 1.40 nm in the LLC-MK2 cells (n = 105; p > 0.05). The virus particles, both in vitro and in vivo, were enclosed into cytoplasmic vesicles. In conclusion, the shape, size, and diameter of the AHFV particle lie within the framework of the genus Flavivirus, family Flaviviridae.
胡姆拉出血热病毒（AHFV）是一种新发现的人畜共患黄病毒，1994 - 1995年首次从沙特阿拉伯吉达以南的胡姆拉地区分离出来。随后，该病毒也在沙特阿拉伯的麦加市（2001 - 2003年）和奈季兰（2008 - 2009年）被分离出来。该病毒可引起伴有肝炎、出血表现和脑炎的急性发热性疾病。据报道，住院患者的病死率为25%。尽管已经发表了该病毒的一些生物学和分子特征，但关于该病毒的电子显微镜特征尚无数据。在本文中，我们描述了电子显微镜下AHFV颗粒的形态特征和测量数据，以及病毒颗粒在新生Wistar大鼠脑细胞和恒河猴（猕猴）肾上皮细胞（LLC - MK2）中的定位。LLC - MK2细胞和大鼠脑细胞中的病毒颗粒均显示出深色的六边形核心（衣壳）和半透明的包膜。在大鼠脑细胞（n = 154）中，包膜病毒颗粒的平均直径为40.59±1.29nm，在LLC - MK2细胞（n = 105；p>0.05）中为40.97±1.40nm。病毒颗粒在体外和体内均被包裹在细胞质小泡中。总之，AHFV颗粒的形状、大小和直径均在黄病毒科黄病毒属的范围内。
