Murakami Takaaki, Kamada Kazuhiro, Mizushima Katsura, Higashimura Yasuki, Katada Kazuhiro, Uchiyama Kazuhiko, Handa Osamu, Takagi Tomohisa, Naito Yuji, Itoh Yoshito
Department of Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kyoto, Japan.
Digestion. 2017;95(1):55-60. doi: 10.1159/000452364. Epub 2017 Jan 5.
Irritable bowel syndrome (IBS) causes chronic abdominal pain and abnormal bowel movements. The etiology involves complicated interactions among visceral hypersensitivity, disorders related to bowel movements, and stress. Changes in the microbiota affect the IBS pathophysiology. We investigated changes in colorectal motility, structure, and microbiota in response to stress due to maternal separation (MS) and corticotropin-releasing hormone (CRH) administration in rats.
Neonatal rats were separated from their mothers for 3 h daily from postnatal day (PND) 2 to PND14. The control group included rats of the same age that were not separated. After MS, the rats were undisturbed for 5 weeks. At 8 weeks of age, 10 µg of CRH or saline was intravenously administered to MS and control groups. Two hours later, the number of fecal pellets was counted. Three hours after CRH or saline administration, the rats were sacrificed and colorectal tissue samples and cecal contents were collected to analyze the fecal microbiota. The number of fecal pellets was significantly greater in MS with the CRH group. Both stressors altered the microbiota composition. Key Messages: Among rats that received CRH, MS increased the colorectal motility. Stress due to MS altered the gut microbiota composition.
肠易激综合征（IBS）会导致慢性腹痛和肠道运动异常。其病因涉及内脏高敏感性、与肠道运动相关的紊乱以及应激之间的复杂相互作用。微生物群的变化会影响IBS的病理生理学。我们研究了大鼠因母婴分离（MS）和注射促肾上腺皮质激素释放激素（CRH）而产生应激时，结肠运动性、结构和微生物群的变化。
从出生后第2天（PND）到PND14，每天将新生大鼠与其母亲分离3小时。对照组包括未分离的同龄大鼠。MS后，大鼠在5周内不受干扰。8周龄时，向MS组和对照组静脉注射10μg CRH或生理盐水。两小时后，计算粪便颗粒数量。在注射CRH或生理盐水3小时后，处死大鼠并收集结肠组织样本和盲肠内容物以分析粪便微生物群。CRH处理的MS组粪便颗粒数量显著更多。两种应激源均改变了微生物群组成。关键信息：在接受CRH的大鼠中，MS增加了结肠运动性。MS引起的应激改变了肠道微生物群组成。
