He X, Ma Y, Yi G, Wu J, Zhou L, Guo H
Public Research Laboratory, Hainan Medical University, Haikou, China.
School of Pharmacy, Hebei Medical University, Shijiazhuang, China.
Lett Appl Microbiol. 2017 May;64(5):350-354. doi: 10.1111/lam.12711. Epub 2017 Mar 19.
In recent years, the incidence of clinical yeast infections has increased dramatically. Due to the extensive use of broad-spectrum antifungal agents, there has been a notable increase in drug resistance among infections yeast species. As one of the most popular natural antimicrobial agents, essential oils (EOs) have attracted a lot of attention from the scientific community. The aim of this study was to analyse the chemical composition and examine the antifungal activity of the EO extracted from the seeds of Carica papaya Linn. The papaya seed EO was analysed by gas chromatography-mass spectrometry. The major constituent is benzyl isothiocyanate (99·36%). The filter paper disc diffusion method and broth dilution method were employed. The EO showed inhibitory effect against all the tested Candida strains including C. albicans, C. glabrata, C. krusei, C. parapsilosis and C. tropical with inhibition zone diameters in the range of 14·2-33·2 mm, the minimal inhibitory concentrations (MICs) in the range of 4·0-16·0 μg ml and the minimum fungicidal concentrations (MFCs) in the range of 16·0-64·0 μg ml . Here, we found that the papaya seed EO has promising anticandida activity and identify C. papaya L. as a potential natural source of antifungal agents.
The chemical composition and antifungal activity of essential oil of Carica papaya seeds were studied. The oil of papaya seeds could inhibit the growth of Candida spp. for the first report. Carica Papaya may be recognized as a possible new source of natural antifungal agents.
近年来，临床酵母菌感染的发生率急剧上升。由于广谱抗真菌药物的广泛使用，感染酵母菌种类的耐药性显著增加。作为最受欢迎的天然抗菌剂之一，精油（EOs）已引起科学界的广泛关注。本研究的目的是分析番木瓜种子提取的精油的化学成分，并检测其抗真菌活性。采用气相色谱 - 质谱联用仪分析番木瓜种子精油。主要成分是异硫氰酸苄酯（99.36%）。采用滤纸片扩散法和肉汤稀释法。该精油对所有测试的念珠菌菌株包括白色念珠菌、光滑念珠菌、克柔念珠菌、近平滑念珠菌和热带念珠菌均有抑制作用，抑菌圈直径在14.2 - 33.2毫米范围内，最小抑菌浓度（MICs）在4.0 - 16.0微克/毫升范围内，最小杀菌浓度（MFCs）在16.0 - 64.0微克/毫升范围内。在此，我们发现番木瓜种子精油具有良好的抗念珠菌活性，并确定番木瓜为潜在的天然抗真菌剂来源。
研究了番木瓜种子精油的化学成分和抗真菌活性。首次报道番木瓜种子油可抑制念珠菌属的生长。番木瓜可能被认为是一种可能的新型天然抗真菌剂来源。
