David Jebaraj D, Utsumi Hideo, Milton Franklin Benial A
Department of Physics, The American College, Madurai, 625 002, Tamilnadu, India.
Innovation Center for Medical Redox Navigation, Kyushu University, Fukuoka, 812 8582, Japan.
Magn Reson Chem. 2017 Aug;55(8):700-705. doi: 10.1002/mrc.4576. Epub 2017 Feb 1.
The electron spin resonance studies were carried out for 2 mm concentration of N-labeled and N-labeled 3-carbamoyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl, 3-carboxy-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl, 3-methoxycarbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl and their deuterated nitroxyl radicals using X-band electron spin resonance spectrometer. The electron spin resonance line shape analysis was carried out. The electron spin resonance parameters such as linewidth, Lorentzian component, signal intensity ratio, rotational correlation time, hyperfine coupling constant and g-factor were estimated. The deuterated nitroxyl radicals have narrow linewidth and an increase in Lorentzian component, compared with undeuterated nitroxyl radicals. The dynamic nuclear polarization factor was observed for all nitroxyl radicals. Upon H labeling, about 70% and 40% increase in dynamic nuclear polarization factor were observed for N-labeled and N-labeled nitroxyl radicals, respectively. The signal intensity ratio and g-value indicate the isotropic nature of the nitroxyl radicals in pure water. Therefore, the deuterated nitroxyl radicals are suitable spin probes for in vivo/in vitro electron spin resonance and Overhauser-enhanced magnetic resonance imaging modalities. Copyright © 2017 John Wiley & Sons, Ltd.
使用X波段电子自旋共振光谱仪对2 mM浓度的N标记和N标记的3-氨基甲酰基-2,2,5,5-四甲基吡咯烷-1-氧基、3-羧基-2,2,5,5-四甲基吡咯烷-1-氧基、3-甲氧基羰基-2,2,5,5-四甲基吡咯烷-1-氧基及其氘代硝酰自由基进行了电子自旋共振研究。进行了电子自旋共振线形状分析。估计了电子自旋共振参数，如线宽、洛伦兹分量、信号强度比、旋转相关时间、超精细耦合常数和g因子。与未氘代的硝酰自由基相比，氘代硝酰自由基具有更窄的线宽和增加的洛伦兹分量。观察到所有硝酰自由基的动态核极化因子。在进行H标记时，N标记和N标记的硝酰自由基的动态核极化因子分别增加了约70%和40%。信号强度比和g值表明了硝酰自由基在纯水中的各向同性性质。因此，氘代硝酰自由基是适用于体内/体外电子自旋共振和奥弗豪泽增强磁共振成像模式的自旋探针。版权所有© 2017约翰·威利父子有限公司。
