Department of Clinical Medical Research, The Second Clinical Medical College of Jinan University (Shenzhen People's Hospital), Shenzhen, China.
Nephrology Department of Guilin 181st Hospital, Guangxi Key Laboratory of Metabolic Diseases Research, Guilin, China.
Genes Immun. 2017 Jan;18(1):22-27. doi: 10.1038/gene.2016.45. Epub 2017 Jan 5.
Systemic lupus erythematosus (SLE) is a systemic autoimmune disease that is known to be associated with polyclonal B-cell hyper-reactivity. B-cell receptor (BCR) has a central role in B-cell development, activation, survival and apoptosis, and thus is a critical component of the regulation of both protective and autoreactive B cells. In this study, we applied multiplex PCR and Illumina high-throughput sequencing to study the composition and variation of the BCRs in peripheral blood mononuclear cells from SLE patients and healthy donors (NC). We found that SLE group displayed significantly shorter CDR3 average length (14.86±0.76aa vs 15.70±0.43aa), more arginine percentage of CDR3 amino acids (7.57±0.20% vs 7.32±0.19%) and poorer immunological diversity than the healthy ones. CDR3 sequence YGMDV present in all SLE samples may provide more information in generating more effective B-cell targeted diagnosis/therapies strategies.
系统性红斑狼疮（SLE）是一种全身性自身免疫性疾病，已知与多克隆 B 细胞过度反应有关。B 细胞受体（BCR）在 B 细胞的发育、激活、存活和凋亡中起着核心作用，因此是调节保护性和自身反应性 B 细胞的关键组成部分。在这项研究中，我们应用多重 PCR 和 Illumina 高通量测序来研究 SLE 患者和健康供体（NC）外周血单个核细胞中 BCR 的组成和变异。我们发现 SLE 组的 CDR3 平均长度明显更短（14.86±0.76aa 对 15.70±0.43aa），CDR3 氨基酸中的精氨酸百分比更高（7.57±0.20% 对 7.32±0.19%），免疫多样性更差。所有 SLE 样本中存在的 CDR3 序列 YGMDV 可能提供更多信息，从而生成更有效的 B 细胞靶向诊断/治疗策略。
