Monos D S, Bina M, Kahn S N
Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia 19104.
Clin Biochem. 1989 Oct;22(5):369-71. doi: 10.1016/s0009-9120(89)80034-7.
We examined different antisera and varying serum dilutions for their effects on the sensitivity of immunofixation electrophoresis (IFE) for detecting and characterizing serum IgG paraproteins. Different antisera reacted unequally with a single paraprotein and conversely, a single antiserum reacted unequally with several different paraproteins. The dilution of serum was extremely important; the adoption of a single dilution for all sera could result in failure to detect low-concentration or poorly immunoreactive paraproteins. While the application of a standardized amount of paraprotein is recommended, it must be emphasized that no single antiserum can be relied on to provide unequivocal detection of all paraproteins of that particular isotype.
我们检测了不同抗血清及不同血清稀释度对免疫固定电泳（IFE）检测和鉴定血清IgG副蛋白敏感性的影响。不同抗血清与单一副蛋白的反应不同，反之，单一抗血清与几种不同副蛋白的反应也不同。血清稀释极为重要；对所有血清采用单一稀释度可能导致无法检测到低浓度或免疫反应性差的副蛋白。虽然建议使用标准化量的副蛋白，但必须强调的是，不能依赖单一抗血清明确检测出该特定同种型的所有副蛋白。
