Tözsér J, Berta A, Punyiczki M
Department of Biochemistry, Medical University of Debrecen, Hungary.
Clin Chim Acta. 1989 Aug 31;183(3):323-31. doi: 10.1016/0009-8981(89)90367-7.
Plasminogen activator activity and plasminogen independent amidolytic activity were measured in human tears by a spectrophotometric method using human plasminogen and chromogenic peptide substrate S-2251. This assay is sensitive predominantly to urokinase-like plasminogen activator. Tears were collected with glass capillaries. The activator activity in normal tears was found to be low, 0.06 +/- 0.04 (SD) IU/ml. Elevated levels were measured in the tears of patients with various types of conjunctival and corneal disorders. The affected epithelial cells of the cornea and conjunctiva were suggested to be responsible for the elevated activity. Plasminogen independent amidolytic activity was usually very low except in cases of increased permeability of the conjunctival blood vessels. The procedure is recommended as a useful tool for the study of the pathological changes in the epithelial cells of the cornea and conjunctiva.
采用分光光度法，使用人纤溶酶原和发色肽底物S - 2251，测定人泪液中的纤溶酶原激活剂活性和非纤溶酶原依赖性酰胺水解活性。该检测方法主要对尿激酶样纤溶酶原激活剂敏感。用玻璃毛细管收集泪液。发现正常泪液中的激活剂活性较低，为0.06±0.04（标准差）IU/ml。在患有各种类型结膜和角膜疾病的患者泪液中检测到活性升高。角膜和结膜受影响的上皮细胞被认为是活性升高的原因。除结膜血管通透性增加的情况外，非纤溶酶原依赖性酰胺水解活性通常非常低。该方法被推荐为研究角膜和结膜上皮细胞病理变化的有用工具。
