测量生物流体小（纳升）液滴中液体接界电位的方法。

Method of measuring liquid junction potentials in small (nanolitre) droplets of biological fluids.

Sugo E, Györy A Z

Department of Medicine, Sydney University, New South Wales, Australia.

Clin Exp Pharmacol Physiol. 1989 Jul;16(7):555-60. doi: 10.1111/j.1440-1681.1989.tb01605.x.

Abstract

A method is described which allows the determination of liquid junction potentials between biological fluid samples of less than 1 nL with a reproducibility of 10%. This was achieved by use of a micro-method and the use of 3% agarose added to the electrolyte in the measuring electrodes. 2. Validation was performed with a modified flowing boundary method and the system was also tested using a solution containing divalent ions. Liquid junction potential between surface fluid from rat kidneys and harvested renal proximal tubular fluids was -0.62 +/- 0.11 mV (mean +/- s.d., n = 66).

摘要

本文描述了一种方法，该方法能够测定体积小于1 nL的生物流体样本之间的液体接界电位，重现性为10%。这是通过使用一种微方法以及在测量电极的电解质中添加3%的琼脂糖来实现的。2. 采用改进的流动边界法进行了验证，并且还使用含有二价离子的溶液对该系统进行了测试。大鼠肾脏表面流体与采集的肾近端小管液之间的液体接界电位为-0.62±0.11 mV（平均值±标准差，n = 66）。