Mohan M S, Bates R G
Clin Chem. 1975 Jun;21(7):864-72.
We measured electromotive force at 37 degrees C with pH, sodium glass, valinomycin, and the Simon calcium electrodes in synthetic electrolyte mixtures simulating serum, in cells both with and without liquid junction. Evidently these electrodes respond in a near-Nernstian manner to changes in concentration of the ion sensed. We su-gest that such mixtures may serve as calibrating standards for ion-selective electrodes in clinical analysis. The effect of ionic strength on electrode response can be accounted for almost wholly by alterations in the activity coefficients. The lanthanum fluoride electrode activity coefficients. The lanthanum fluoride electrode shows promise as a reliable reference in cells without liquid junction.
我们在37摄氏度下，使用pH电极、钠玻璃电极、缬氨霉素电极和西蒙钙电极，在模拟血清的合成电解质混合物中，在有和没有液接界的细胞中测量电动势。显然，这些电极对所检测离子浓度的变化以近能斯特方式作出响应。我们建议，此类混合物可作为临床分析中离子选择电极的校准标准。离子强度对电极响应的影响几乎完全可以通过活度系数的变化来解释。氟化镧电极的活度系数。氟化镧电极有望成为无液接界细胞中可靠的参比电极。
