Graduate Program in Dentistry, Federal University of Pelotas, Pelotas, RS, Brazil.
J Oral Pathol Med. 2017 Sep;46(8):632-636. doi: 10.1111/jop.12546. Epub 2017 Jan 28.
To compare histologically and imaginologically the bone structure of rats' mandibles treated with bisphosphonates (BPs) and rats that did not receive BPs.
Thirty-four rat specimens (Rattus novergicus, Wistar strain) were divided into three groups: (i) 12 rats treated with zoledronic acid; (ii) 12 rats treated with clodronate; and (iii) the control group, containing 10 rats that received saline solution. All individuals were exposed to cone beam computed tomography (CBCT). The images were processed and analyzed to obtain the Hounsfield scores, using the software OsiriX 7.0. Sixty-eight histological slides were obtained from the specimens and stained with hematoxylin and eosin (HE). Using the software Adobe Photoshop CS6, the histological areas containing non-vital bone were identified and quantified.
Non-vital bone presented positive association with the zoledronic acid and clodronate groups. Statistically, no significant difference in bone density was observed among the groups.
Based on the results, the BP therapy alone was sufficient to induce osteonecrosis. In addition, the CBCT was not a sensible method for detection of the early signs of bone modification in individuals under BP therapy.
比较接受和未接受双膦酸盐（BPs）治疗的大鼠下颌骨的组织学和影像学骨结构。
34 个大鼠标本（Wistar 大鼠）被分为三组：（i）12 只接受唑来膦酸治疗的大鼠；（ii）12 只接受氯膦酸治疗的大鼠；（iii）对照组，包含 10 只接受生理盐水治疗的大鼠。所有大鼠均接受锥形束 CT（CBCT）检查。使用 OsiriX 7.0 软件处理和分析图像，以获得 Hounsfield 评分。从标本中获得 68 张组织学切片，并用苏木精和伊红（HE）染色。使用 Adobe Photoshop CS6 软件，识别和量化含有非活力骨的组织学区域。
非活力骨与唑来膦酸和氯膦酸组呈正相关。统计学分析显示，各组之间的骨密度无显著差异。
根据结果，单独使用 BP 治疗足以诱导骨坏死。此外，CBCT 不是检测 BP 治疗个体早期骨改建迹象的敏感方法。
