Delanora Leonardo Alan, de Lima Neto Tiburtino José, da Rocha Tiago Esgalha, Silveira Glauco Rodrigues Carmo, Levin Liran, Shibli Jamil Awad, Ervolino Edilson, Mourão Carlos Fernando, Faverani Leonardo P
Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University (UNESP), Araçatuba 16015-050, Brazil.
Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba 13414-903, Brazil.
Biomedicines. 2025 May 20;13(5):1248. doi: 10.3390/biomedicines13051248.
: Medication-related osteonecrosis of the jaw (MRONJ) is a challenging condition often associated with bisphosphonate use, leading to impaired bone healing and difficult clinical management. Given the lack of predictable therapeutic options, this study investigated the effects of systemic ozone therapy on MRONJ healing. This study aimed to analyze the effects of systemic ozone therapy on oral hard and soft tissue healing in senescent rats with medication-related osteonecrosis of the jaw (MRONJ) induced by antiresorptive therapy. : Twenty-eight senescent Wistar rats, aged eighteen months and weighing ~350 g, were used for this study. The animals were divided into four groups. The negative control (SAL) group received saline applications, while the control-treated (SAL+OZ) group received saline applications and ozone therapy (0.7 mg/kg). The MRONJ (ZOL) group received Zoledronate, an intravenous antiresorptive drug (100 μg/kg), and the MRONJ-treated (ZOL+OZ) group received zoledronate application and was treated with systemic ozone therapy (0.7 mg/kg). All rats underwent molar extraction in the third week of the experiment and were euthanized in the seventh week of the experiment. The mandibles were resected, reduced, and prepared for microtomographic analysis, histopathological/histometric analysis, and immunohistochemistry. : The ZOL group presented characteristics of vitreous, non-vital, and dense bone, poor vascularization, and high values of inflammation markers compatible with MRONJ. In contrast, the ZOL+OZ group exhibited improvement in alveolar bone and soft tissue healing, a decrease in nonvital bone area, and modulation of local inflammation. : It can be concluded that Ozone therapy improved oral hard and soft tissue healing of MRONJ in senescent female rats subjected to antiresorptive drugs and might be considered for future clinical applications.
药物相关性颌骨坏死（MRONJ）是一种具有挑战性的病症，常与双膦酸盐的使用相关，会导致骨愈合受损及临床管理困难。鉴于缺乏可预测的治疗选择，本研究调查了全身臭氧疗法对MRONJ愈合的影响。本研究旨在分析全身臭氧疗法对经抗吸收治疗诱导的药物相关性颌骨坏死（MRONJ）老年大鼠口腔软硬组织愈合的影响。：本研究使用了28只18个月大、体重约350克的老年Wistar大鼠。动物被分为四组。阴性对照组（SAL）接受生理盐水涂抹，而对照治疗组（SAL+OZ）接受生理盐水涂抹及臭氧疗法（0.7毫克/千克）。MRONJ组（ZOL）接受唑来膦酸，一种静脉抗吸收药物（100微克/千克），而MRONJ治疗组（ZOL+OZ）接受唑来膦酸涂抹并接受全身臭氧疗法（0.7毫克/千克）。所有大鼠在实验的第三周进行磨牙拔除，并在实验的第七周实施安乐死。切除下颌骨，进行处理，准备用于显微断层扫描分析、组织病理学/组织计量学分析及免疫组织化学分析。：ZOL组呈现出玻璃样、无活力、致密骨的特征，血管化不良，且炎症标志物值高，符合MRONJ表现。相比之下，ZOL+OZ组在牙槽骨和软组织愈合方面有所改善，无活力骨面积减少，局部炎症得到调节。：可以得出结论，臭氧疗法改善了接受抗吸收药物治疗的老年雌性大鼠MRONJ的口腔软硬组织愈合，未来临床应用中可考虑使用。
