Larrauri Alexander, Núñez Oscar, Hernández-Cassou Santiago, Saurina Javier
University of Barcelona, Department of Analytical Chemistry, Martí i Franquès 1-11, E-08028, Barcelona, Spain.
J AOAC Int. 2017 Mar 1;100(2):323-329. doi: 10.5740/jaoacint.16-0407. Epub 2017 Jan 5.
The determination of polyphenols in wines is of great interest in the field of food analysis due to health and organoleptic implications. In addition, the applicability of polyphenols as food descriptors to be used for characterization, classification, and authentication purposes is gaining popularity. In this work, a simple and reliable method based on HPLC separation in reversed-phase mode with UV-Vis detection was developed and applied to determine polyphenolic compounds in white wines. The chromatographic separation was performed using a C18 column under a methanol elution gradient and assessed by an experimental design approach. Analytical parameters were established under the optimal experimental conditions. LOD values were between 3 and 220 µg/L, and repeatability values were better than 1% for most of the analyzed polyphenols. Compositional data were further exploited to characterize white wines based on principal component analysis to discriminate among mono- and polyvarietal compositions.
由于健康和感官方面的影响，葡萄酒中多酚的测定在食品分析领域备受关注。此外，多酚作为用于表征、分类和认证目的的食品描述符的适用性正日益受到欢迎。在这项工作中，开发了一种基于反相模式下HPLC分离并结合紫外可见检测的简单可靠方法，并将其应用于测定白葡萄酒中的多酚化合物。使用C18柱在甲醇洗脱梯度下进行色谱分离，并通过实验设计方法进行评估。在最佳实验条件下确定了分析参数。检测限（LOD）值在3至220μg/L之间，对于大多数分析的多酚，重复性值优于1%。进一步利用成分数据基于主成分分析对白葡萄酒进行表征，以区分单品种和多品种成分。
