Xu Weisheng, Li Dong, Liu Jing, Yang Yuexin
National Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing, China.
Wei Sheng Yan Jiu. 2013 May;42(3):497-503, 528.
To develop a novel quantitative method for simultaneous analysis of 11 polyphenols in ratafee by reversed-phase high performance liquid chromatography.
The separation was performed on a Agilent Eclipse XDB C18 (4.6 mmx250 mm, 51 m) and the column temperature was set at 30 degrees C. The mobile phase was consisted of 0. 1% phosphoric acid (A) and methanol (B), with a linear gradient elution at a flow rate of I. 0 ml/min. The gradient program was as follow: 10% B isocratic (0-10 min), 10%-20% B liner (10-15 min), 20% B isocratic (15-25 min), 20%-60% B liner (25-65 min), 60% B isocratic (65-75 min). And detection wavelength was 210, 270, 320, 327 and 360 nm.
The correlation coefficients of the 11 calibration curves were all higher than 0.9995. The scopes of the recovery rate was within the range of 85.1% to 112.0%, with RSDs no more than 2%. The limit of quantification (LOQ) and the detection limit (LOD) were 0.32-1.59 ng and 0.10-0.48 ng, respectively.
The method is accurate and reliable, and can successfully analyze different ratafee samples from different sources.
建立一种采用反相高效液相色谱法同时分析果酒中11种多酚的新型定量方法。
采用安捷伦Eclipse XDB C18色谱柱（4.6 mm×250 mm，5μm）进行分离，柱温设定为30℃。流动相由0.1%磷酸（A）和甲醇（B）组成，以1.0 ml/min的流速进行线性梯度洗脱。梯度程序如下：10% B等度洗脱（0 - 10 min），10% - 20% B线性梯度洗脱（10 - 15 min），20% B等度洗脱（15 - 25 min），20% - 60% B线性梯度洗脱（25 - 65 min），60% B等度洗脱（65 - 75 min）。检测波长为210、270、320、327和360 nm。
11条校准曲线的相关系数均高于0.9995。回收率范围在85.1%至112.0%之间，相对标准偏差不超过2%。定量限（LOQ）和检测限（LOD）分别为0.32 - 1.59 ng和0.10 - 0.48 ng。
该方法准确可靠，能够成功分析不同来源的果酒样品。
