Sauvain J-J, Suarez G, Edmé J-L, Bezerra O M P A, Silveira K G, Amaral L S, Carneiro A P S, Chérot-Kornobis N, Sobaszek A, Hulo S
Institute for Work and Health, University of Lausanne and Geneva, Epalinges-Lausanne, Switzerland.
J Breath Res. 2017 Jan 24;11(1):016010. doi: 10.1088/1752-7163/aa56dd.
A particle exposure assessment based on the dose deposited in the lungs would be the gold standard for the evaluation of any resulting health effects. Measuring particles in exhaled breath condensate (EBC)-a matrix containing water and airway lining fluid-could help to evaluate particle retention in the lungs. This study aimed to (1) validate a nanoparticle tracking analysis (NTA) method for determining the particle number concentration and their hydrodynamic size distribution in EBC, and (2) apply this method to EBC collected from workers exposed to soapstone (n = 55) or quartz dust (n = 12) and controls (n = 11). A standard latex bead solution was used to determine the linear range, limit of detection (LOD), repeatability (coefficient of variation, CV), and bias in spiked EBC. An LM10 NanoSight instrument with NTA version 3.1 software was used for measurement. RTubes were used for field collection of EBC. The repeatability obtained for a D size distribution in EBC showed less than 8% variability, with a bias <7%. The particle concentration was linear in the range ≤2.5 × 10 particles ml with a LOD of 4 × 10 particles ml. A recovery of 117 ± 20% at 6.2 × 10 particles ml was obtained with a CV <10% and a bias <20%. EBC from workers exposed to quartz, who experienced the largest exposure to silica particles, consistently exhibited a statistically significant (p < 0.01) higher concentration of particles in their EBC, with a size distribution shift towards larger values than the other groups. Results showed that the NTA technique performed well for characterizing the size distribution and concentrations of particles in EBC. The technique needs to be corroborated with a larger population of workers.
基于沉积在肺部的剂量进行的颗粒物暴露评估将是评估任何由此产生的健康影响的金标准。测量呼出气冷凝物（EBC，一种含有水和气道内衬液的基质）中的颗粒物，有助于评估肺部的颗粒物滞留情况。本研究旨在：（1）验证一种纳米颗粒跟踪分析（NTA）方法，用于测定EBC中的颗粒数浓度及其流体动力学尺寸分布；（2）将该方法应用于从接触滑石（n = 55）或石英粉尘（n = 12）的工人以及对照组（n = 11）收集的EBC。使用标准乳胶珠溶液确定加标EBC中的线性范围、检测限（LOD）、重复性（变异系数，CV）和偏差。使用配备NTA 3.1版软件的LM10纳米可视分析仪进行测量。使用RTubes进行EBC的现场采集。EBC中D尺寸分布的重复性显示变异率小于8%，偏差<7%。颗粒浓度在≤2.5×10颗粒/ml范围内呈线性，LOD为4×10颗粒/ml。在6.2×10颗粒/ml时回收率为117±20%，CV<10%，偏差<20%。接触石英的工人的EBC，他们接触二氧化硅颗粒的程度最大，其EBC中的颗粒浓度始终呈现出统计学上显著（p<0.01）的更高水平，且尺寸分布向比其他组更大的值偏移。结果表明，NTA技术在表征EBC中颗粒的尺寸分布和浓度方面表现良好。该技术需要在更多工人中得到验证。
