通过扫描电子显微镜（SEM）和能量色散X射线微分析（EDX）检测禽输卵管分泌颗粒中的钙
Calcium Detection in Secretion Granules of Avian Oviduct by Scanning Electron Microscopy (SEM) and Energy-dispersive X-Ray Microanalysis (EDX) .
作者信息
Makita Takashi, Hatsuoka Masanori, Sugi Yukiko
机构信息
Department of Veterinary Anatomy (T.M; M.H.), Yamaguchi University, Yamaguchi City, and Department of Anatomy (Y.S.), Saitama Medical College, Japan (OA 82-281P1).
出版信息
J Histochem Cytochem. 1983 Jan;31(1A_suppl):219-221. doi: 10.1177/31.1A_SUPPL.6827073.
Secretion granules in the shell gland, isthmus, and albumin-secreting region of the hen oviduct were analyzed with WET-scanning electron microscopy (SEM) and EDX, a combination of wide-angle backscattered electron detector (BED) and energy-dispersive X-ray microanalyzer (EDX). Glutaraldehyde-fixed but unhydrated, unstained, and uncoated samples were analyzed; Ca was localized in all secretion granules in all three sections of the hen oviduct studied.
采用湿扫描电子显微镜（SEM）和能谱仪（EDX）（广角背散射电子探测器（BED）和能量色散X射线微分析仪（EDX）的组合）对母鸡输卵管的壳腺、峡部和白蛋白分泌区域的分泌颗粒进行了分析。对经戊二醛固定但未水化、未染色和未镀膜的样本进行了分析；在所研究的母鸡输卵管的所有三个部位的所有分泌颗粒中均检测到了钙的存在。
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