Roels Frank, Pauwels Marina, Cornelis Alfons, Kerckaert Ingrid, Spek Peter Van Der, Goovaerts Gerda, Versieck Jacques, Goldfischer Sidney
Menselijki Anatomie (F.R.; M.P.; A.C.; I.K.; P. v.d.S.), Gastroenterologie. Akademisch Ziekenhuis (G.G.), and Patologische Ontleedkunde (J.V.), Vrije Universiteit Brussel, 1090 Brussels, Belgium; Gastroenterologie (J.V.). Rijksuniversiteit Gent: and Department of Pathology and Liver Research Center (S.G.), Albert Einstein College of Medicine, Bronx, New York 10461 (OA 82-287P2).
J Histochem Cytochem. 1983 Jan;31(1A_suppl):235-237. doi: 10.1177/31.1A_SUPPL.6186727.
The number, intracellular distribution, and staining characteristics of human hepatocellular peroxisomes that had been made visible by cytochemical staining for catalase were evaluated in biopsies from 75 patients with hepatic, inflammatory, or malignant disease and ten normal individuals. Intensity of staining was found to be proportional to enzymatic activity by microspectrophotometry. Scanning transmission electron microscopy (STEM) image analysis demonstrated an inverse relationship between peroxisomal size and contrast. Peroxisomes were more abundant, and often concentrated in a perinuclear configuration in cholestatic and cirrhotic livers. Decreased peroxisomal staining was common in cholestasis, cirrhosis, hepatitis, and in almost all patients with malignancies, both with and without hepatic metastases.
