人类肝脏中的过氧化物酶体（微体）：85例活检标本的细胞化学和定量研究。

Peroxisomes (microbodies) in human liver: cytochemical and quantitative studies of 85 biopsies.

作者信息

Roels F, Pauwels M, Cornelis A, Kerckaert I, Van der Spek P, Goovaerts G, Versieck J, Goldfischer S

出版信息

J Histochem Cytochem. 1983 Jan;31(1A Suppl):235-7.

Abstract

The number, intracellular distribution, and staining characteristics of human hepatocellular peroxisomes that had been made visible by cytochemical staining for catalase were evaluated in biopsies from 75 patients with hepatic, inflammatory, or malignant disease and ten normal individuals. Intensity of staining was found to be proportional to enzymatic activity by microspectrophotometry. Scanning transmission electron microscopy (STEM) image analysis demonstrated an inverse relationship between peroxisomal size and contrast. Peroxisomes were more abundant, and often concentrated in a perinuclear configuration in cholestatic and cirrhotic livers. Decreased peroxisomal staining was common in cholestasis, cirrhosis, hepatitis, and in almost all patients with malignancies, both with and without hepatic metastases.

摘要

通过过氧化氢酶细胞化学染色使人类肝细胞过氧化物酶体可见后，对75例患有肝脏、炎症或恶性疾病的患者及10名正常个体的活检样本中过氧化物酶体的数量、细胞内分布及染色特征进行了评估。通过显微分光光度法发现染色强度与酶活性成正比。扫描透射电子显微镜（STEM）图像分析表明过氧化物酶体大小与对比度呈负相关。在胆汁淤积性和肝硬化肝脏中，过氧化物酶体更为丰富，且常呈核周分布。在胆汁淤积、肝硬化、肝炎以及几乎所有恶性肿瘤患者（无论有无肝转移）中，过氧化物酶体染色减少都很常见。