Sar Madhabananda, Stumpf Walter E
Departments of Anatomy and Pharmacology, University of North Carolina, Chapel Hill, North Carolina 27514.
J Histochem Cytochem. 1981 Jan;29(1A_suppl):161-166. doi: 10.1177/29.1A_SUPPL.6895229.
A combined technique of autoradiography and immunohistochemistry is described for localization of radioactively labeled ligands and antibodies to neuropeptides, protein hormones and neurotransmitter synthesizing enzymes in the brain. This method permits the simultaneous visualization of radioactively labeled cells and neuropeptide-producing cells in the same tissue preparation. Autoradiograms are fixed with weak paraformaldehyde solution prior to photographic processing for subsequent immunoperoxidase staining. With this procedure 3H-estradiol is localized in neurophysin I and arginine vasopressin-producing cells of the mouse supraoptic and paraventricular nuclei, neurophysin-producing cells of the guinea pig hypothalamus, and dopamine-β-hydroxylase-containing neurons of the rat lower brainstem; 3H-dihydrotestosterone in pituitary gonadotropes and thyrotropes; and 3H-1,25-(OH)2 vitamin D3 in pituitary thyrotropes and pancreatic B-cells.
本文描述了一种放射自显影术与免疫组织化学相结合的技术，用于在大脑中定位放射性标记的配体以及针对神经肽、蛋白质激素和神经递质合成酶的抗体。该方法能够在同一切片标本中同时观察到放射性标记的细胞和产生神经肽的细胞。在进行后续免疫过氧化物酶染色的照相处理之前，先用弱多聚甲醛溶液固定放射自显影片。通过此方法，在小鼠视上核和室旁核中产生神经垂体素I和精氨酸加压素的细胞、豚鼠下丘脑产生神经垂体素的细胞以及大鼠脑干下部含多巴胺-β-羟化酶的神经元中定位到了³H-雌二醇；在垂体促性腺激素细胞和促甲状腺激素细胞中定位到了³H-双氢睾酮；在垂体促甲状腺激素细胞和胰腺β细胞中定位到了³H-1,25-(OH)₂维生素D₃。
