Kim Y S, Sar M, Stumpf W E
Cell Tissue Res. 1979 May 25;198(3):435-40. doi: 10.1007/BF00234188.
The distribution of estradiol-concentrating cells in the pituitary of the platyfish, Xiphophorus maculatus, is studied after the injection of 3H estradiol-17 beta by thaw-mount autoradiography. Autoradiograms prepared 2-8 h after the injection show nuclear concentration of radioactivity in certain cells of the rostral pars distalis, pars intermedia and pars nervosa. Radioactively labeled cells are identified as gonadotropes by a combined technique of thaw-mount autoradiography and immunoperoxidase staining with antiserum to ovine LH. Approximately 80% of the immunoreactive LH cells show a concentration of radioactivity in their nuclei. These observations indicate that in teleosts, as in mammals, estradiol has a direct effect on pituitary gonadotropes.
通过冷冻切片放射自显影术，研究了在注射³H-17β-雌二醇后，剑尾鱼（Xiphophorus maculatus）垂体中雌二醇浓缩细胞的分布情况。注射后2 - 8小时制备的放射自显影片显示，在吻端远侧部、中间部和神经部的某些细胞中存在放射性核素的核内聚集。通过冷冻切片放射自显影术和用抗羊促黄体生成素抗血清进行免疫过氧化物酶染色的联合技术，将放射性标记细胞鉴定为促性腺激素细胞。大约80%的免疫反应性促黄体生成素细胞在其细胞核中显示出放射性聚集。这些观察结果表明，在硬骨鱼中，如同在哺乳动物中一样，雌二醇对垂体促性腺激素细胞有直接作用。
