Beckman W C
Department of Anatomy, University of North Carolina, Chapel Hill, North Carolina 27514.
J Histochem Cytochem. 1981 Jan;29(1A_suppl):212-215. doi: 10.1177/29.1A_SUPPL.7288158.
Automated systems for the evaluation of autoradiograms were compared with the traditional method of manual counting. The automated systems included televison image analysis systems, reflectance/densitometric systems, and semiautomatic digitizing pad systems. Highly automated analysis systems were considered applicable for quantitation of autoradiograms when the subcellular location of the radioactivity is not an important consideration, when background is low, and when operator interaction with the system can be kept at a minimum. In many cases the relationship between silver grains and cell compartments is important for interpretation of the results. Under these conditions, the most rapid, reproducible and reliable method would combine measurement of cellular dimensions with a digitizing pad and manual counting of silver grains by the observer. Such a system should be connected to a computer for formatting and statistical evaluation of the data. Initial testing of a quantitative autoradiographic technique indicated that the autoradiographic slide is a reliable estimator of the radioactivity present in the tissue of experimental animals injected with tritium labeled compounds. Accuracy in counting was lost when silver grains became coincident. Thus, a system for the quantitation of silver grains in relation to cellular morphology requires careful selection of exposure time, computer assisted measurement of cellular dimensions, operator evaluation of silver grains and computerized statistical analysis of the data.
对用于评估放射自显影片的自动化系统与传统的手动计数方法进行了比较。自动化系统包括电视图像分析系统、反射率/密度测量系统和半自动数字化平板系统。当放射性的亚细胞定位不是重要考虑因素、背景较低且操作员与系统的交互可保持在最低限度时，高度自动化的分析系统被认为适用于放射自显影片的定量分析。在许多情况下，银颗粒与细胞区室之间的关系对于结果的解释很重要。在这些条件下，最快速、可重复且可靠的方法是将细胞尺寸的测量与数字化平板相结合，并由观察者手动计数银颗粒。这样的系统应连接到计算机，以便对数据进行格式化和统计评估。对一种定量放射自显影技术的初步测试表明，放射自显影片是注射了氚标记化合物的实验动物组织中存在的放射性的可靠估计指标。当银颗粒重合时，计数准确性会丧失。因此，一个用于定量与细胞形态相关的银颗粒的系统需要仔细选择曝光时间、计算机辅助测量细胞尺寸、操作员对银颗粒进行评估以及对数据进行计算机化统计分析。
