Suissa M
Anal Biochem. 1983 Sep;133(2):511-4. doi: 10.1016/0003-2697(83)90117-3.
Autoradiograms or fluorograms can be quantified by eluting the silver grains from the developed film with 1 M NaOH and measuring the absorbance of the eluate in a conventional spectrometer. In contrast to direct scanning of the film, the present method is rapid, requires only simple equipment, and permits accurate quantitation of even heavily exposed bands.
放射自显影片或荧光照片可以通过用1M氢氧化钠从显影后的胶片上洗脱银颗粒，并在传统光谱仪中测量洗脱液的吸光度来进行定量。与直接扫描胶片相比，本方法快速，仅需简单设备，并且即使对于曝光过度的条带也能进行准确的定量。
