College of Resources and Environmental Engineering, Wuhan University of Technology, Wuhan 430070, PR China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China.
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, PR China.
Bioresour Technol. 2017 Mar;228:39-46. doi: 10.1016/j.biortech.2016.12.065. Epub 2016 Dec 22.
A bioelectrochemically-assisted constructed wetland (BECW) system was used to treat nitrate-contaminated wastewater without organic carbon source. The denitrification performance and microbial community composition of a BECW in closed-circuit mode (BECW-C) was compared to a BECW in open-circuit mode (BECW-O). The highest denitrification efficiency (78.92±3.12%) was obtained in the BECW-C with an applied current of 15mA. No nitrite accumulation was observed during the autotrophic denitrification process in the BECW-C. The significantly higher denitrification efficiency of the BECW-C compared to the BECW-O suggested enhanced denitrification due to in situ generation of hydrogen. The bacterial communities in the anode, cathode and rhizosphere regions collected from the BECW-C (with 10 or 15mA) and the BECW-O were characterized using 16S rRNA pyrosequencing technology, which revealed different microbial community structures among the treatments. The results also indicated that Thiohalophilus and Clostridium sensu stricto might be responsible for autotrophic denitrification in the BECW-C.
采用无有机碳源的生物电化学辅助人工湿地（BECW）系统处理硝酸盐污染废水。比较了闭路（BECW-C）和开路（BECW-O）两种模式下 BECW 的反硝化性能和微生物群落组成。在施加电流为 15mA 的 BECW-C 中获得了最高的反硝化效率（78.92±3.12%）。在 BECW-C 的自养反硝化过程中未观察到亚硝酸盐积累。与 BECW-O 相比，BECW-C 的反硝化效率显著提高，这表明由于原位产生氢气，反硝化作用得到了增强。采用 16S rRNA 焦磷酸测序技术对 BECW-C（施加 10 或 15mA）和 BECW-O 的阳极、阴极和根际区域采集的细菌群落进行了表征，结果表明不同处理之间的微生物群落结构存在差异。结果还表明，Thiohalophilus 和 Clostridium sensu stricto 可能负责 BECW-C 中的自养反硝化。
