16S rRNA 测序揭示了处理盐芥菜废水的同步硝化反硝化和有机物去除工艺中的细菌群落结构。
Bacterial community structure in simultaneous nitrification, denitrification and organic matter removal process treating saline mustard tuber wastewater as revealed by 16S rRNA sequencing.
机构信息
Faculty of Urban Construction and Environmental Engineering, Chongqing University, Chongqing 400045, PR China.
Faculty of Urban Construction and Environmental Engineering, Chongqing University, Chongqing 400045, PR China; Key Laboratory of the Three Gorges Reservoir's Eco-Environments, Ministry of Education, Chongqing University, Chongqing 400045, PR China.
出版信息
Bioresour Technol. 2017 Mar;228:31-38. doi: 10.1016/j.biortech.2016.12.071. Epub 2016 Dec 23.
A simultaneous nitrification, denitrification and organic matter removal (SNDOR) process in sequencing batch biofilm reactor (SBBR) was established to treat saline mustard tuber wastewater (MTWW) in this study. An average COD removal efficiency of 86.48% and total nitrogen removal efficiency of 86.48% were achieved at 30gNaClL during 100days' operation. The underlying mechanisms were investigated by PacBio SMRT DNA sequencing (V1-V9) to analyze the microbial community structures and its variation from low salinity at 10gNaClL to high salinity at 30gNaClL. Results showed elevated salinity did not affect biological performance but reduced microbial diversity in SBBR, and halophilic bacteria gradually predominated by succession. Despite of high C/N, autotrophic ammonia-oxidizing bacteria (AOB) Nitrosomonas and ammonia-oxidizing archaea (AOA) Candidatus Nitrososphaera both contributed to ammonium oxidation. As salinity increasing, nitrite-oxidizing bacteria (NOB) were significantly inhibited, partial nitrification and denitrification (PND) process gradually contributed to nitrogen removal.
本研究采用序批式生物膜反应器（SBBR）构建同步硝化反硝化及有机物去除（SNDOR）工艺，处理榨菜加工废水（MTWW）。在 30gNaCl/L 条件下运行 100 天，COD 去除率和总氮去除率分别达到 86.48%和 86.48%。通过 PacBio SMRT DNA 测序（V1-V9）分析微生物群落结构及其从低盐 10gNaCl/L 到高盐 30gNaCl/L 的变化，探讨了其内在机制。结果表明，高盐度并未影响 SBBR 的生物性能，但降低了微生物多样性，嗜盐菌逐渐取代其他微生物成为优势菌群。尽管 C/N 较高，但自养氨氧化菌（AOB）硝化单胞菌和氨氧化古菌（AOA）Candidatus Nitrososphaera 均有助于氨氮氧化。随着盐度的升高，亚硝酸盐氧化菌（NOB）受到明显抑制，部分硝化反硝化（PND）过程逐渐对脱氮起作用。
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