Ji Chang Yoon, Chung Won-Hyong, Kim Ho Soo, Jung Won Yong, Kang Le, Jeong Jae Cheol, Kwak Sang-Soo
Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Daejeon, 34141, South Korea; Department of Green Chemistry and Environmental Biotechnology, Korea University of Science and Technology (UST), 217 Gajeong-ro, Daejeon, 34113, South Korea.
Division of Nutrition and Metabolism Research, Korea Food Research Institute, 1201-62 Anyangpangyo-ro, Sungnam-Si, 13539, South Korea.
Plant Physiol Biochem. 2017 Mar;112:97-108. doi: 10.1016/j.plaphy.2016.12.021. Epub 2016 Dec 24.
Sweetpotato [Ipomoea batatas (L.) Lam] is a globally important root crop with high industrial value. However, because sweetpotato tuberous roots undergo chilling injuries that negatively affect their quality at temperatures below 10 °C, postharvest damage during the winter season is a major constraint for industrialization. To understand chilling injury response during postharvest low temperature storage, we used next-generation sequencing technology to comprehensive analyze the transcriptome of tuberous roots stored at optimal (13 °C) or low temperature (4 °C) for 6 weeks. From nine cDNA libraries, we produced 298,765,564 clean reads, which were de novo assembled into 58,392 unigenes with an average length of 1100 bp. A total of 3216 differentially expressed genes (DEGs) were detected and categorized into six clusters, of which clusters 2, 4, and 5 (1464 DEGs) were up-regulated under low temperature. The genes in these three clusters are involved in biosynthesis of unsaturated fatty acids, pathogen defense, and phenylalanine metabolism. By contrast, genes in clusters 1, 3, and 6 (1752 DEGs), which were generally down-regulated at low temperature, encode antioxidant enzymes or are involved in glycerophospholipid, carbohydrate, or energy metabolism. We confirmed the results of the transcriptome analysis by quantitative RT-PCR. Our transcriptome analysis will advance our understanding of the comprehensive mechanisms of chilling injury during low temperature storage and facilitate improvements in postharvest storage of sweetpotato tuberous roots.
甘薯[Ipomoea batatas (L.) Lam]是一种具有重要全球意义且具有高产业价值的块根作物。然而，由于甘薯块根在温度低于10°C时会遭受冷害，这对其品质产生负面影响，因此冬季收获后的损伤是产业化的主要制约因素。为了解收获后低温储存期间的冷害响应，我们使用下一代测序技术全面分析了在最佳温度（13°C）或低温（4°C）下储存6周的块根转录组。从9个cDNA文库中，我们获得了298,765,564条clean reads，这些序列被从头组装成58,392个单基因，平均长度为1100 bp。共检测到3216个差异表达基因（DEGs），并将其分为六个簇，其中簇2、4和5（共1464个DEGs）在低温下上调。这三个簇中的基因参与不饱和脂肪酸的生物合成、病原体防御和苯丙氨酸代谢。相比之下，簇1、3和6中的基因（共1752个DEGs）在低温下通常下调，这些基因编码抗氧化酶或参与甘油磷脂、碳水化合物或能量代谢。我们通过定量RT-PCR证实了转录组分析的结果。我们的转录组分析将加深我们对低温储存期间冷害综合机制的理解，并有助于改善甘薯块根的收获后储存。
