Tang Wei, Arisha Mohamed Hamed, Zhang Zhenyi, Yan Hui, Kou Meng, Song Weihan, Li Chen, Gao Runfei, Ma Meng, Wang Xin, Zhang Yungang, Li Zongyun, Li Qiang
Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District/Sweetpotato Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Breeding of Sweetpotato, Ministry of Agriculture and Rural Affairs, Xuzhou, China.
Institute of Integrative Plant Biology, Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics, School of Life Sciences, Jiangsu Normal University, Xuzhou, China.
Front Plant Sci. 2023 Jan 19;13:1081948. doi: 10.3389/fpls.2022.1081948. eCollection 2022.
Crops are affected by various abiotic stresses, among which heat (HT) and drought (DR) stresses are the most common in summer. Many studies have been conducted on HT and DR, but relatively little is known about how drought and heat combination (DH) affects plants at molecular level.
Here, we investigated the responses of sweetpotato to HT, DR, and DH stresses by RNA-seq and data-independent acquisition (DIA) technologies, using controlled experiments and the quantification of both gene and protein levels in paired samples.
Twelve cDNA libraries were created under HT, DR, and DH conditions and controls. We identified 536, 389, and 907 DEGs in response to HT, DR, and DH stresses, respectively. Of these, 147 genes were common and 447 were specifically associated with DH stress. Proteomic analysis identified 1609, 1168, and 1535 DEPs under HT, DR, and DH treatments, respectively, compared with the control, of which 656 were common and 358 were exclusive to DH stress. Further analysis revealed the DEGs/DEPs were associated with heat shock proteins, carbon metabolism, phenylalanine metabolism, starch and cellulose metabolism, and plant defense, amongst others. Correlation analysis identified 6465, 6607, and 6435 co-expressed genes and proteins under HT, DR, and DH stresses respectively. In addition, a combined analysis of the transcriptomic and proteomic data identified 59, 35, and 86 significantly co-expressed DEGs and DEPs under HT, DR, and DH stresses, respectively. Especially, top 5 up-regulated co-expressed DEGs and DEPs (At5g58770, C24B11.05, Os04g0679100, BACOVA_02659 and HSP70-5) and down-regulated co-expressed DEGs and DEPs (AN3, PMT2, TUBB5, FL and CYP98A3) were identified under DH stress.
This is the first study of differential genes and proteins in sweetpotato under DH stress, and it is hoped that the findings will assist in clarifying the molecular mechanisms involved in sweetpotato resistance to heat and drought stress.
作物受到多种非生物胁迫的影响，其中高温（HT）和干旱（DR）胁迫是夏季最常见的。关于高温和干旱已经开展了许多研究，但对于干旱和高温复合胁迫（DH）如何在分子水平上影响植物，人们了解得相对较少。
在此，我们通过RNA测序和数据非依赖采集（DIA）技术，利用对照实验以及对配对样本中基因和蛋白质水平的定量分析，研究了甘薯对高温、干旱和复合胁迫的响应。
在高温、干旱、复合胁迫条件及对照条件下构建了12个cDNA文库。我们分别鉴定出536个、389个和907个响应高温、干旱和复合胁迫的差异表达基因（DEG）。其中，147个基因是共同的，447个基因与复合胁迫特异性相关。蛋白质组学分析分别鉴定出与对照相比，在高温、干旱和复合胁迫处理下有1609个、1168个和1535个差异表达蛋白（DEP），其中656个是共同的，358个是复合胁迫所特有的。进一步分析表明，这些差异表达基因/差异表达蛋白与热休克蛋白、碳代谢、苯丙氨酸代谢、淀粉和纤维素代谢以及植物防御等有关。相关性分析分别鉴定出在高温、干旱和复合胁迫下有6465个、6607个和6435个共表达的基因和蛋白质。此外，对转录组和蛋白质组数据的联合分析分别鉴定出在高温、干旱和复合胁迫下有59个、35个和86个显著共表达的差异表达基因和差异表达蛋白。特别是，在复合胁迫下鉴定出了上调程度最高的前5个共表达差异表达基因和差异表达蛋白（At5g58770、C24B11.05、Os04g0679100、BACOVA_02659和HSP70 - 5）以及下调程度最高的前5个共表达差异表达基因和差异表达蛋白（AN3、PMT2、TUBB5、FL和CYP98A3）。
这是关于甘薯在复合胁迫下差异基因和蛋白质的首次研究，希望这些发现将有助于阐明甘薯耐热和耐旱胁迫的分子机制。
