Xuzhou Institute of Agricultural Sciences of the Xuhuai District of Jiangsu Province, Xuzhou, Jiangsu, 221131, P.R. China.
School of Food and Biological Engineering, Hefei University of Technology, Hefei, Anhui, 230009, P.R. China.
Sci Rep. 2019 Jul 31;9(1):11141. doi: 10.1038/s41598-019-47604-8.
The activities and gene expression of antioxidative enzymes and the ROS content were analyzed in two typical storage-tolerant cultivars (Xushu 32 and Shangshu 19) and another two storage-sensitive cultivars (Yanshu 25 and Sushu 16) to explore the association between the storage capacity of sweetpotato (Ipomoea batatas (L.) Lam) and ROS scavenging capability. The storage roots of the storage-tolerant cultivars maintained higher activities and expression levels of antioxidative enzymes, including ascorbate peroxidase (APX), peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD); lower activity and expression of lipoxygenase (LOX); and lower accumulation of ROS metabolites compared with the storage-sensitive cultivars. The antioxidative capability and ROS parameters of leaves were positively correlated with those of storage roots. Our results provide valuable insight for evaluating the storability of sweetpotato cultivars by analyzing the capabilities of the antioxidative system and the contents of ROS metabolites.
分析了两种典型耐贮品种（徐薯 32 和商薯 19）和另外两种敏感品种（烟薯 25 和苏薯 16）的抗氧化酶活性和基因表达以及活性氧（ROS）含量，以探讨甘薯（Ipomoea batatas（L.）Lam）贮藏能力与 ROS 清除能力之间的关系。耐贮品种的贮藏根保持较高的抗氧化酶活性和表达水平，包括抗坏血酸过氧化物酶（APX）、过氧化物酶（POD）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）；较低的脂氧合酶（LOX）活性和表达；ROS 代谢物的积累也低于敏感品种。叶片的抗氧化能力和 ROS 参数与贮藏根的抗氧化能力和 ROS 参数呈正相关。通过分析抗氧化系统的能力和 ROS 代谢物的含量，我们的结果为评估甘薯品种的贮藏性提供了有价值的见解。
