Institute for Food and Agricultural Technology (INTEA), University of Girona, Campus Montilivi (EPS-1), Girona, 17003, Spain.
Mol Plant Pathol. 2018 Feb;19(2):418-431. doi: 10.1111/mpp.12534. Epub 2017 Mar 15.
Fruit crops are regarded as important health promoters and constitute a major part of global agricultural production, and Rosaceae species are of high economic impact. Their culture is threatened by bacterial diseases, whose control is based on preventative treatments using compounds of limited efficacy and negative environmental impact. One of the most economically relevant examples is the pathogen Xanthomonas arboricola pv. pruni (Xap) affecting Prunus spp. The plant immune response against pathogens can be triggered and amplified by plant elicitor peptides (Peps), perceived by specific receptors (PEPRs). Although they have been described in various angiosperms, scarce information is available on Rosaceae species. Here, we identified the Pep precursor (PROPEP), Pep and PEPR orthologues of 10 Rosaceae species and confirmed the presence of the Pep/PEPR system in this family. We showed the perception and elicitor activity of Rosaceae Peps using the Prunus-Xap pathosystem as proof-of-concept. Treatment with nanomolar doses of Peps induced the corresponding PROPEP and a set of defence-related genes in Prunus leaves, and enhanced resistance against Xap. Peps from the same species had the highest efficiencies. Rosaceae Peps could potentially be used to develop natural, targeted and environmentally friendly strategies to enhance the resistance of Prunus species against biotic attackers.
水果作物被认为是重要的健康促进者，构成了全球农业生产的主要部分，而蔷薇科物种具有很高的经济影响力。它们的栽培受到细菌性疾病的威胁，这些疾病的控制基于使用效果有限且对环境有负面影响的化合物进行预防性处理。最具经济相关性的例子之一是影响李属植物的病原菌黄单胞菌属杨树溃疡亚种（Xap）。植物对病原体的免疫反应可以被植物激发子肽（Peps）触发和放大，这些肽被特定的受体（PEPRs）感知。尽管它们在各种被子植物中都有描述，但蔷薇科物种的信息却很少。在这里，我们鉴定了 10 种蔷薇科物种的 Pep 前体（PROPEP）、Pep 和 PEPR 直系同源物，并证实了该家族中存在 Pep/PEPR 系统。我们使用李属植物-Xap 病理系统作为概念验证，展示了蔷薇科 Pep 的感知和激发子活性。用纳摩尔剂量的 Pep 处理诱导李属植物叶片中相应的 PROPEP 和一组防御相关基因，并增强了对 Xap 的抗性。来自同一物种的 Pep 效率最高。蔷薇科 Pep 可能被用于开发天然、靶向和环保的策略，以增强李属植物对生物攻击的抗性。
