Yoshino Tomoko, Tanaka Tsuyoshi, Nakamura Seita, Negishi Ryo, Shionoiri Nozomi, Hosokawa Masahito, Matsunaga Tadashi
Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo, 184-8588, Japan.
Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo, 184-8588, Japan.
Anal Biochem. 2017 Mar 1;520:16-21. doi: 10.1016/j.ab.2016.12.026. Epub 2017 Jan 3.
A cell entrapment device consisting of a microcavity array was used to analyze the deformability of MCF-10 human breast epithelial and MCF-7 human breast cancer cell lines by confocal laser scanning microscopy. Entrapment of up to 8 × 10 cells was achieved within 3 min. Protrusions were formed at the bottom surface of the array with a pore size of 3 μm. Protrusion length increased at higher filtration pressures and could be used to distinguish between MCF-7 and MCF-10 cells. These results indicate that our system is useful for high-throughput deformability analysis of cancer cells, which can provide insight into the mechanisms underlying tumor cell malignancy.
一种由微腔阵列组成的细胞捕获装置，通过共聚焦激光扫描显微镜用于分析MCF-10人乳腺上皮细胞系和MCF-7人乳腺癌细胞系的可变形性。在3分钟内实现了高达8×10个细胞的捕获。在孔径为3μm的阵列底面形成了突起。在较高的过滤压力下突起长度增加，可用于区分MCF-7和MCF-10细胞。这些结果表明，我们的系统对于癌细胞的高通量可变形性分析是有用的，这可以深入了解肿瘤细胞恶性的潜在机制。
