通过微腔阵列评估癌细胞的可变形性。

Evaluation of cancer cell deformability by microcavity array.

作者信息

Yoshino Tomoko, Tanaka Tsuyoshi, Nakamura Seita, Negishi Ryo, Shionoiri Nozomi, Hosokawa Masahito, Matsunaga Tadashi

机构信息

Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo, 184-8588, Japan.

出版信息

Anal Biochem. 2017 Mar 1;520:16-21. doi: 10.1016/j.ab.2016.12.026. Epub 2017 Jan 3.

DOI:10.1016/j.ab.2016.12.026

PMID:28057503

Abstract

A cell entrapment device consisting of a microcavity array was used to analyze the deformability of MCF-10 human breast epithelial and MCF-7 human breast cancer cell lines by confocal laser scanning microscopy. Entrapment of up to 8 × 10 cells was achieved within 3 min. Protrusions were formed at the bottom surface of the array with a pore size of 3 μm. Protrusion length increased at higher filtration pressures and could be used to distinguish between MCF-7 and MCF-10 cells. These results indicate that our system is useful for high-throughput deformability analysis of cancer cells, which can provide insight into the mechanisms underlying tumor cell malignancy.

摘要

一种由微腔阵列组成的细胞捕获装置，通过共聚焦激光扫描显微镜用于分析MCF-10人乳腺上皮细胞系和MCF-7人乳腺癌细胞系的可变形性。在3分钟内实现了高达8×10个细胞的捕获。在孔径为3μm的阵列底面形成了突起。在较高的过滤压力下突起长度增加，可用于区分MCF-7和MCF-10细胞。这些结果表明，我们的系统对于癌细胞的高通量可变形性分析是有用的，这可以深入了解肿瘤细胞恶性的潜在机制。