Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria 3086, Australia.
Sci Rep. 2017 Jan 6;7:39846. doi: 10.1038/srep39846.
Apoptotic bodies (ApoBDs) are membrane-bound extracellular vesicles that can mediate intercellular communication in physiological and pathological settings. By combining recently developed analytical strategies with fluorescence-activated cell sorting (FACS), we have developed a method that enables the isolation of ApoBDs from cultured cells to 99% purity. In addition, this approach also enables the identification and isolation of cell type-specific ApoBDs from tissue, bodily fluid and blood-derived samples.
凋亡小体（ApoBDs）是一种具有膜结合的细胞外囊泡，可以在生理和病理环境中介导细胞间通讯。通过结合最近开发的分析策略和荧光激活细胞分选（FACS），我们开发了一种方法，能够从培养细胞中分离出纯度达到 99%的凋亡小体。此外，这种方法还能够鉴定和分离来自组织、体液和血液样本的特定细胞类型的凋亡小体。
