Toll样受体4在乙型肝炎病毒感染中的抗病毒作用：一项研究。

Anti-viral role of toll like receptor 4 in hepatitis B virus infection: An study.

作者信息

Das Dipanwita, Sarkar Neelakshi, Sengupta Isha, Pal Ananya, Saha Debraj, Bandopadhyay Manikankana, Das Chandrima, Narayan Jimmy, Singh Shivram Prasad, Chakravarty Runu

机构信息

Dipanwita Das, Neelakshi Sarkar, Ananya Pal, Debraj Saha, Manikankana Bandopadhyay, Runu Chakravarty, ICMR Virus Unit, Kolkata, ID and BG Hospital Campus, Kolkata 700010, India.

出版信息

World J Gastroenterol. 2016 Dec 21;22(47):10341-10352. doi: 10.3748/wjg.v22.i47.10341.

AIM

Toll like receptors plays a significant anti-viral role in different infections. The aim of this study was to look into the role of toll like receptor 4 (TLR4) in hepatitis B virus (HBV) infection.

METHODS

Real time PCR was used to analyze the transcription of TLR4 signaling molecules, cell cycle regulators and HBV DNA viral load after triggering the HepG2.2.15 cells with TLR4 specific ligand. Nuclear factor (NF)-κB translocation on TLR4 activation was analyzed using microscopic techniques. Protein and cell cycle analysis was done using Western Blot and FACS respectively.

RESULTS

The present study shows that TLR4 activation represses HBV infection. As a result of HBV suppression, there are several changes in host factors which include partial release in G1/S cell cycle arrest and changes in host epigenetic marks. Finally, it was observed that anti-viral action of TLR4 takes place through the NF-κB pathway.

CONCLUSION

The study shows that TLR4 activation in HBV infection brings about changes in hepatocyte microenvironment and can be used for developing a promising therapeutic target in future.