Bolat Seda Eymen, Ozdemirci Safak, Kasapoglu Taner, Duran Bulent, Goktas Levent, Karahanoglu Ertugrul
Department of Obstetrics and Gynecology, Yalvac State Hospital, Yalvac, Isparta, Turkey.
Department of Gynecology, Etlik Zubeyde Hanim Women's Health Training and Research Hospital, Ankara, Turkey.
North Clin Istanb. 2016 Nov 25;3(2):90-96. doi: 10.14744/nci.2016.02418. eCollection 2016.
The objective of this study was to evaluate the relationship between oocyte yield, fertilization, and clinical pregnancy (CP), and anti-Mullerian hormone (AMH) level in serum and follicular fluid during in vitro fertilization treatment.
Forty-four infertile women who underwent IVF treatment using multiagonist protocol were included in this study. Baseline level of AMH in serum and follicular fluid was measured on third day of menstrual cycle. AMH level in serum and follicular fluid was then measured again on day of oocyte pick-up. Pearson correlation and binary regression tests were used for statistical analysis. For Type 1 error, p=5% was selected as cut-off value for statistical significance.
Serum AMH level was positively correlated with total number of oocytes retrieved and rate of fertilization and CP (r=0.397, p=0.008; r=0.401, p=0.007; and r=0.382, p=0.011, respectively). There was significantly negative correlation between serum level of follicle-stimulating hormone (FSH) and fertilization rate (r=-0.320; p=0.034), as well as serum FSH level and CP rate (r=-0.308; p=0.042). There were no significant correlations between AMH level in follicular fluid and IVF treatment outcomes.
Serum AMH levels may be more reliable for prediction of total number of oocytes retrieved and rate of fertilization and CP than AMH levels in follicular fluid.
本研究旨在评估体外受精治疗期间，卵母细胞产量、受精情况及临床妊娠（CP）与血清和卵泡液中抗苗勒管激素（AMH）水平之间的关系。
本研究纳入了44例采用多激动剂方案进行体外受精治疗的不孕女性。在月经周期的第三天测量血清和卵泡液中AMH的基线水平。然后在取卵日再次测量血清和卵泡液中的AMH水平。采用Pearson相关性检验和二元回归检验进行统计分析。对于I型错误，选择p = 5%作为具有统计学意义的临界值。
血清AMH水平与回收的卵母细胞总数、受精率及临床妊娠率呈正相关（r分别为0.397，p = 0.008；r为0.401，p = 0.007；r为0.382，p = 0.011）。血清卵泡刺激素（FSH）水平与受精率（r = -0.320；p = 0.034）以及血清FSH水平与临床妊娠率（r = -0.308；p = 0.042）之间存在显著负相关。卵泡液中的AMH水平与体外受精治疗结局之间无显著相关性。
血清AMH水平在预测回收的卵母细胞总数、受精率及临床妊娠率方面可能比卵泡液中的AMH水平更可靠。
