在体外受精过程中，卵丘颗粒细胞中组蛋白去甲基化酶 KDM4A 和 KDM4B 的表达。
Histone demethylase KDM4A and KDM4B expression in granulosa cells from women undergoing in vitro fertilization.
机构信息
Institute for Reproductive Health and Regenerative Medicine, Center for Reproductive Sciences, University of Kansas Medical Center, 3901 Rainbow Blvd. MS3050, Kansas City, KS, 66160, USA.
Department of Obstetrics and Gynecology, University of Kansas Medical Center, Kansas City, KS, 66160, USA.
出版信息
J Assist Reprod Genet. 2018 Jun;35(6):993-1003. doi: 10.1007/s10815-018-1151-3. Epub 2018 Mar 14.
PURPOSE
To assess expression of the histone demethylases KDM4A and KDM4B in granulosa collected from women undergoing oocyte retrieval and to determine if expression was related to pregnancy outcome.
METHODS
Cumulus and mural granulosa cells were obtained from women undergoing oocyte retrieval. KDM4A and KDM4B mRNA expression was determined by qRT-PCR. KDM4A and KDM4B proteins were immunohistochemically localized in ovarian tissue sections obtained from archival specimens.
RESULTS
KDM4A and KDM4B protein was localized to oocytes, granulosa cells, and theca and luteal cells in ovaries from reproductive-aged women. KDM4A and KDM4B mRNA expression was overall higher in cumulus compared to mural granulosa. When comparing granulosa demethylase gene expression, KDM4A and KDM4B mRNA expression was higher in both cumulus and mural granulosa from not pregnant patients compared to patients in the pregnant-live birth group.
CONCLUSIONS
Histone demethylases KDM4A and KDM4B mRNA are differentially expressed in cumulus and mural granulosa. Expression of both KDM4A and KDM4B mRNA was lower in cumulus granulosa and mural granulosa from pregnant compared to not pregnant patients. These findings suggest that altered expression of histone demethylases may impact epigenetic changes in granulosa cells associated with pregnancy.
目的
评估组蛋白去甲基酶 KDM4A 和 KDM4B 在接受卵母细胞采集的女性的颗粒细胞中的表达，并确定其表达是否与妊娠结局有关。
方法
从接受卵母细胞采集的女性中获取卵丘和壁颗粒细胞。通过 qRT-PCR 测定 KDM4A 和 KDM4B mRNA 的表达。通过免疫组织化学定位在从存档标本获得的卵巢组织切片中定位 KDM4A 和 KDM4B 蛋白。
结果
KDM4A 和 KDM4B 蛋白定位于生殖年龄女性卵巢中的卵母细胞、颗粒细胞、膜和黄体细胞。与壁颗粒细胞相比，KDM4A 和 KDM4B mRNA 在卵丘中总体表达更高。比较颗粒细胞去甲基酶基因表达时，与妊娠活产组患者相比，未怀孕患者的卵丘和壁颗粒细胞中的 KDM4A 和 KDM4B mRNA 表达均更高。
结论
组蛋白去甲基酶 KDM4A 和 KDM4B mRNA 在卵丘和壁颗粒细胞中差异表达。与未怀孕患者相比，怀孕患者的卵丘颗粒细胞和壁颗粒细胞中的 KDM4A 和 KDM4B mRNA 表达均较低。这些发现表明，组蛋白去甲基酶的表达改变可能会影响与妊娠相关的颗粒细胞中的表观遗传变化。
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