College of Chemistry, Jilin University, Changchun, 130012, China.
Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.
J Am Soc Mass Spectrom. 2017 Mar;28(3):428-433. doi: 10.1007/s13361-016-1584-9. Epub 2017 Jan 5.
We report a novel strategy to achieve simultaneous one-step in-situ self-desalting and enrichment (OISE) of peptides/proteins on a facilely fabricated patterned MALDI steel plate with a circular paraffin-steel-polystyrene structure. The OISE plate could efficiently segregate salts from both analytes and matrices while retaining both analyte and matrix concentrate, and facilitating them to form homogeneous co-crystals on the centrally located polystyrene pattern. With the OISE plate, high quality and reproducible spectra could be obtained for low abundance peptides even in the presence of high salt concentrations (200 mM NHHCO, 1 M NaCl, or 400 mM urea). Using this strategy, a significant sensitivity enhancement was gained over traditional MALDI plate. The practical utility of this method was further demonstrated by the successful profiling of BSA digests and human serum. Graphical Abstract ᅟ.
我们报告了一种新策略，可在具有圆形石蜡-钢-聚苯乙烯结构的简易图案化 MALDI 钢板上实现肽/蛋白质的一步原位自除盐和浓缩（OISE）。OISE 板可有效地将盐从分析物和基质中分离出来，同时保留分析物和基质的浓缩物，并促进它们在位于中心的聚苯乙烯图案上形成均匀的共晶。使用 OISE 板，即使在高盐浓度（200 mM NHHCO、1 M NaCl 或 400 mM 尿素）存在下，也可以为低丰度肽获得高质量和重现性的光谱。与传统的 MALDI 板相比，该策略可显著提高灵敏度。该方法的实际应用通过成功分析 BSA 消化物和人血清进一步证明。
