Department of Chemistry, Fudan University, Shanghai 200433, China.
State Key Laboratory of ASIC and System, Fudan University, Shanghai 200433, China.
Talanta. 2017 Dec 1;175:427-434. doi: 10.1016/j.talanta.2017.07.078. Epub 2017 Jul 25.
In this work, a TiO film was prepared on a MALDI plate by atomic layer deposition (ALD) technique and then modified with -NH. The obtained TiO-NH modified plate was applied for on-plate simultaneous enrichment of phosphopeptides and glycopeptides. The ALD TiO film displayed quite uniform morphology, and attached firmly to the MALDI plate with rather stable physical and chemical properties, which resulted in fine stability of the plate in performance. The -NH groups offered the film better hydrophilicity and affinity toward glycopeptides. The on-plate simultaneous enrichment performance of the TiO-NH modified plate was investigated by β-casein digests, HRP digests and human serum.
在这项工作中，通过原子层沉积（ALD）技术在 MALDI 板上制备了 TiO 薄膜，然后用 -NH 进行修饰。所得的 TiO-NH 修饰板用于板上同时富集磷酸肽和糖肽。ALD TiO 薄膜显示出相当均匀的形态，并且与 MALDI 板牢固地附着，具有相当稳定的物理和化学性质，从而使板的性能具有良好的稳定性。-NH 基团使薄膜具有更好的亲水性和亲糖肽性。通过β-酪蛋白消化物、HRP 消化物和人血清研究了 TiO-NH 修饰板的板上同时富集性能。
